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Sample GSM13272 Query DataSets for GSM13272
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 23
Sample type RNA
 
Channel 1
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 48h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_034
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.414384355 90 40 0 47 0 1.575 52 324 42 65 0 90 86 0 116 0 1.506 52 312 307 312 0 100 395 0 679 0 1.783 80 404 1835 1946 0
A#10 -0.162223683 80 40 0 47 0 1.23 52 237 158 191 0 80 77 0 110 0 1.243 52 258 1040 1052 0 80 342 0 639 0 1.219 52 263 6995 7416 0
A#100 -0.72965092 80 47 0 54 0 2.306 52 217 29 44 0 70 132 0 155 0 1.898 32 188 202 227 0 70 684 0 963 0 1.698 32 188 1548 1791 0
A#1000 0.026351147 100 54 0 66 0 1.452 80 288 74 95 0 90 169 0 215 0 1.349 52 273 513 532 0 90 1009 0 1367 0 1.281 52 245 3428 3630 0
A#10000 -0.120214964 110 82 0 92 0 1.389 80 215 88 112 0 110 297 0 295 0 1.376 80 222 711 720 0 110 2089 0 2312 0 1.352 80 219 4556 4397 0
A#10001 0.210931879 100 82 0 94 0 1.412 80 244 111 170 0 110 292 0 286 0 1.416 80 209 959 1045 0 100 2222 0 2413 0 1.315 80 228 4528 6224 0
A#10002 0.447184908 110 88 0 102 0 1.237 80 268 154 247 0 120 322 0 330 0 1.139 120 198 5028 8815 0 110 2622 0 2647 0 1.273 80 258 6577 8924 0
A#10003 0.016142015 100 88 0 111 0 1.241 80 249 182 225 0 110 366 0 429 0 1.683 80 200 498 612 0 100 2487 0 2858 0 1.244 80 271 8472 8438 0
A#10004 -0.209122618 110 89 0 114 0 1.164 80 295 401 513 0 110 370 0 434 0 2.417 80 213 289 328 0 110 2494 0 2807 0 1.159 80 285 17881 19442 0
A#10005 0.313876284 110 86 0 108 0 1.269 80 244 132 212 0 100 398 0 426 0 3.614 80 211 -28 115 -50 110 2334 0 2721 0 1.297 80 252 5977 8304 0
A#10006 0.165806039 110 84 0 102 0 1.175 80 228 284 367 0 120 428 0 428 0 1.153 120 219 3733 5559 0 110 2160 0 2613 0 1.179 80 236 12303 13532 0
A#10007 -0.070463173 110 81 0 100 0 1.235 80 255 305 399 0 100 388 0 400 0 2.361 80 197 -145 -28 -50 110 2105 0 2529 0 1.23 80 259 14339 14556 0
A#10008 0.423681849 100 83 0 103 0 1.303 80 251 145 241 0 110 372 0 422 0 1.307 80 227 1042 1566 0 100 2174 0 2631 0 1.246 80 251 6788 9134 0
A#10009 100 83 0 103 0 3.343 80 246 4 10 -50 100 370 0 424 0 2.012 80 227 -149 -44 -50 100 2187 0 2633 0 3.301 80 247 172 288 -50
A#1001 0.93224233 100 54 0 68 0 1.852 80 235 64 78 0 90 179 0 218 0 1.733 52 257 304 436 0 100 1081 0 1431 0 1.798 80 343 2382 3071 0
A#10010 -0.181149457 110 81 0 101 0 1.197 80 305 216 237 0 100 389 0 413 0 2.627 80 227 -219 -90 -50 110 2132 0 2620 0 1.245 80 286 9338 9273 0
A#10011 -0.139024907 100 79 0 99 0 1.431 80 279 117 146 0 100 383 0 390 0 2.02 80 209 317 352 0 100 2129 0 2596 0 1.349 80 311 5299 5510 0
A#10012 0.421780956 100 79 0 97 0 1.634 80 212 53 101 0 100 369 0 385 0 2.751 80 255 128 145 -50 80 2124 0 2584 0 1.568 52 189 3035 3955 0
A#10013 100 79 0 97 0 3.781 80 235 -17 0 -50 90 358 0 370 0 1.621 52 224 312 288 0 100 2181 0 2436 0 2.654 80 312 -954 -5 -50
A#10014 0.245778867 90 79 0 97 0 1.633 52 227 68 98 0 100 318 0 347 0 3.057 80 234 16 5 -50 90 2166 0 2338 0 2.004 52 236 2972 4091 0

Total number of rows: 43200

Table truncated, full table size 5775 Kbytes.




Supplementary data files not provided

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