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Sample GSM13273 Query DataSets for GSM13273
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 24
Sample type RNA
 
Channel 1
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 48h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_035
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 55 0 598 0 2.794 80 471 11 127 -50 100 192 0 2891 0 2.876 80 457 67 726 -50 100 1109 0 18319 0 3.04 80 422 710 5551 -50
A#10 -0.002335124 80 40 0 47 0 1.292 52 252 147 202 0 80 82 0 113 0 1.282 52 254 925 1063 0 80 412 0 561 0 1.225 52 263 7278 6692 0
A#100 60 46 0 54 0 1.633 32 152 39 45 0 100 135 0 166 0 1.822 80 280 130 149 -50 60 673 0 847 0 1.685 32 154 2022 1512 0
A#1000 -0.002569534 90 64 0 79 0 1.479 52 289 81 121 0 90 227 0 279 0 1.401 52 285 549 617 0 90 1483 0 1585 0 1.276 52 284 4061 4160 0
A#10000 -0.183451453 100 62 0 73 0 1.284 80 242 139 188 0 110 227 0 257 0 1.257 80 287 988 975 0 110 1399 0 1461 0 1.272 80 303 7008 6256 0
A#10001 0.318212391 110 59 0 71 0 1.383 80 241 106 188 0 110 204 0 242 0 1.283 80 274 703 970 0 110 1310 0 1353 0 1.392 80 266 4925 6439 0
A#10002 0.292284269 110 63 0 76 0 1.169 80 256 235 415 0 110 235 0 264 0 1.177 80 282 1641 2169 0 110 1466 0 1508 0 1.172 80 290 11515 14352 0
A#10003 -0.21375606 100 68 0 90 0 1.224 80 244 171 229 0 100 261 0 338 0 1.228 80 250 1208 1185 0 100 1611 0 2068 0 1.203 80 255 8262 7540 0
A#10004 0.066729765 110 69 0 99 0 1.162 80 251 620 988 0 110 270 0 382 0 1.127 80 245 4215 5160 0 110 1783 0 2382 0 1.102 80 245 30664 33379 0
A#10005 0.259929174 110 71 0 107 0 1.29 80 276 127 227 0 110 286 0 424 0 1.265 80 267 872 1222 0 110 1892 0 2527 0 1.227 80 267 6412 7607 0
A#10006 -0.097845634 100 75 0 110 0 1.119 80 250 647 918 0 100 310 0 439 0 1.089 80 254 4361 4814 0 100 1913 0 2611 0 1.081 80 257 32215 31272 0
A#10007 -0.086269709 110 78 0 108 0 1.12 80 265 461 681 0 110 320 0 420 0 1.123 80 257 3253 3552 0 110 2025 0 2570 0 1.122 80 259 22789 23176 0
A#10008 0.355161875 110 77 0 103 0 1.62 80 262 81 143 0 110 313 0 391 0 1.632 80 260 529 784 0 110 2039 0 2360 0 1.635 80 230 3829 5417 0
A#10009 100 73 0 95 0 2.85 80 246 9 20 -50 100 293 0 361 0 2.636 80 271 38 87 -50 100 1959 0 2089 0 3.161 80 214 421 660 -50
A#1001 0.399173216 90 65 0 76 0 2.115 52 303 53 92 0 90 238 0 270 0 1.943 52 303 355 499 0 90 1465 0 1581 0 1.564 52 303 2880 3585 0
A#10010 -0.389757498 120 67 0 87 0 1.416 120 260 231 265 0 120 260 0 314 0 1.283 120 264 1598 1420 0 120 1739 0 1831 0 1.231 120 238 11733 9389 0
A#10011 -0.300208766 110 65 0 82 0 1.269 80 271 197 245 0 110 230 0 287 0 1.298 80 260 1310 1208 0 110 1612 0 1728 0 1.243 80 265 9437 8192 0
A#10012 -0.044233284 80 63 0 79 0 2.18 52 201 31 56 0 90 220 0 273 0 1.849 52 221 217 303 0 80 1512 0 1595 0 2.419 52 190 1236 1959 0
A#10013 100 66 0 81 0 3.181 80 287 4 22 -50 100 232 0 273 0 3.093 80 262 29 131 -50 100 1455 0 1613 0 3.31 80 248 173 846 -50
A#10014 0.042444472 90 66 0 80 0 1.55 52 267 92 128 0 100 235 0 263 0 1.327 80 271 531 687 0 100 1444 0 1547 0 1.37 80 266 3961 4413 0

Total number of rows: 43200

Table truncated, full table size 5747 Kbytes.




Supplementary data files not provided

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