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Sample GSM13276 Query DataSets for GSM13276
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 9
Sample type RNA
 
Channel 1
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_001
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 110 49 0 44 0 2.665 80 461 25 31 0 110 113 0 103 0 2.659 80 444 117 170 0 110 408 0 350 0 3.36 80 511 668 729 0
A#10 -0.217530384 100 47 0 43 0 2.777 80 312 22 27 0 120 103 0 91 0 2.893 120 416 109 151 0 110 353 0 269 0 2.4 80 366 491 689 0
A#100 90 45 0 41 0 2.686 52 272 4 18 -50 90 95 0 85 0 2.46 52 256 27 93 -50 90 324 0 246 0 2.618 52 245 98 417 -50
A#1000 90 49 0 44 0 3.353 52 258 -2 -1 -50 90 97 0 95 0 5.17 52 257 7 -6 -50 90 380 0 291 0 4.203 52 267 -73 -3 -50
A#10000 100 54 0 49 0 1.631 80 254 77 75 0 100 137 0 126 0 1.69 80 239 400 421 0 100 479 0 405 0 1.557 80 233 1972 1846 0
A#10001 70 59 0 51 0 2.121 32 172 31 58 0 90 140 0 119 0 2.789 52 220 162 323 0 90 506 0 386 0 2.029 52 240 934 1388 0
A#10002 0.355973562 110 56 0 51 0 1.546 80 288 93 142 0 110 144 0 130 0 1.432 80 263 488 818 0 110 548 0 443 0 1.306 80 272 2332 3507 0
A#10003 0.098325057 100 56 0 50 0 1.547 80 250 96 134 0 100 143 0 130 0 1.43 80 263 573 798 0 100 506 0 429 0 1.363 80 235 2726 3225 0
A#10004 0.4128051 110 57 0 52 0 1.388 80 255 354 547 0 110 143 0 132 0 1.233 80 281 1976 3217 0 110 498 0 443 0 1.209 80 265 9470 13799 0
A#10005 0.114605129 100 56 0 53 0 1.59 80 252 54 80 0 100 144 0 135 0 1.756 80 242 281 462 0 100 534 0 485 0 1.662 80 256 1591 1937 0
A#10006 0.45533181 100 56 0 52 0 1.247 80 234 250 418 0 100 143 0 130 0 1.256 80 224 1410 2489 0 100 576 0 471 0 1.231 80 237 6872 10254 0
A#10007 0.195351426 110 58 0 53 0 1.228 80 233 335 436 0 110 141 0 134 0 1.202 80 215 1758 2634 0 110 582 0 504 0 1.211 80 221 8981 10719 0
A#10008 -0.132786276 110 60 0 54 0 1.412 80 246 179 185 0 110 156 0 144 0 1.341 80 247 861 1078 0 110 608 0 506 0 1.287 80 241 4239 4581 0
A#10009 60 60 0 57 0 2.542 32 134 28 61 0 70 153 0 150 0 1.892 32 170 155 351 0 70 561 0 514 0 2.33 32 170 961 1478 0
A#1001 -0.236600603 110 50 0 44 0 2.626 80 357 17 20 0 100 101 0 91 0 2.876 80 296 125 137 0 90 374 0 276 0 2.765 52 281 492 564 0
A#10010 -0.23513495 110 56 0 53 0 1.389 80 293 206 207 0 110 144 0 135 0 1.5 80 315 1061 1176 0 110 546 0 475 0 1.349 80 344 5064 4984 0
A#10011 -0.034048884 100 55 0 51 0 1.595 80 272 84 97 0 110 136 0 132 0 1.574 80 270 433 591 0 100 542 0 448 0 1.615 80 277 2198 2455 0
A#10012 70 62 0 53 0 3.045 32 171 29 53 0 80 135 0 127 0 2.266 52 178 170 289 0 70 656 0 488 0 1.977 32 164 729 1081 0
A#10013 90 61 0 53 0 1.735 52 264 79 95 0 110 141 0 125 0 1.685 80 221 413 571 0 100 585 0 462 0 1.68 80 253 2102 2357 0
A#10014 0.190571446 90 58 0 52 0 2.345 52 271 54 83 0 100 129 0 123 0 1.774 80 212 294 477 0 100 486 0 405 0 1.805 80 235 1429 2000 0

Total number of rows: 43200

Table truncated, full table size 5458 Kbytes.




Supplementary data files not provided
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