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Sample GSM13277 Query DataSets for GSM13277
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 10
Sample type RNA
 
Channel 1
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_002
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.283023398 100 41 0 41 0 2.171 80 376 36 45 0 100 94 0 92 0 1.782 80 386 232 253 0 100 307 0 309 0 1.962 80 376 820 1142 0
A#10 -0.277245409 110 41 0 41 0 1.755 80 342 58 67 0 110 93 0 92 0 1.615 80 353 340 407 0 110 290 0 299 0 1.841 80 356 1354 1567 0
A#100 90 52 0 49 0 2.433 52 256 8 32 -50 90 148 0 140 0 3.432 52 250 50 178 -50 90 521 0 495 0 3.695 52 256 265 785 -50
A#1000 90 55 0 51 0 2.737 52 234 15 11 -50 90 172 0 148 0 2.526 52 264 52 72 -50 90 597 0 490 0 2.791 52 258 384 342 -50
A#10000 100 63 0 55 0 1.383 80 241 87 108 0 100 198 0 163 0 1.417 80 237 476 642 0 100 743 0 578 0 1.357 80 223 2051 2602 0
A#10001 100 63 0 53 0 1.556 80 237 60 62 0 100 194 0 151 0 1.697 80 229 351 360 0 100 724 0 529 0 1.506 80 226 1495 1571 0
A#10002 0.413798143 110 65 0 54 0 1.297 80 230 162 135 0 100 203 0 163 0 1.29 80 212 900 796 0 110 777 0 577 0 1.274 80 245 3937 3167 0
A#10003 0.16507694 100 67 0 55 0 1.331 80 212 168 161 0 100 217 0 167 0 1.275 80 210 961 972 0 100 793 0 601 0 1.26 80 239 4488 3921 0
A#10004 0.12487711 110 66 0 56 0 1.157 80 219 578 523 0 110 228 0 173 0 1.135 80 219 3319 3084 0 110 789 0 617 0 1.126 80 232 14114 12504 0
A#10005 -0.175774993 110 66 0 58 0 1.527 80 233 81 96 0 100 231 0 177 0 1.459 80 245 487 574 0 110 804 0 607 0 1.449 80 253 2209 2319 0
A#10006 1.089475935 110 64 0 56 0 1.204 80 241 545 313 0 110 218 0 167 0 1.175 80 254 2973 1844 0 110 784 0 546 0 1.186 80 255 13068 7250 0
A#10007 0.21618232 110 63 0 55 0 1.155 80 211 452 395 0 110 202 0 157 0 1.175 80 210 2655 2317 0 110 759 0 559 0 1.145 80 210 11148 9385 0
A#10008 -0.226949806 110 64 0 54 0 1.249 80 211 209 232 0 110 211 0 151 0 1.28 80 223 1191 1373 0 110 734 0 546 0 1.323 80 211 5058 5478 0
A#10009 90 62 0 54 0 1.506 52 217 64 69 0 90 205 0 151 0 1.571 52 219 345 387 0 80 733 0 512 0 1.429 52 188 1509 1604 0
A#1001 2.082810784 110 52 0 52 0 1.444 80 325 78 66 0 100 146 0 142 0 1.523 80 300 446 391 0 110 537 0 508 0 1.424 80 323 1709 1641 0
A#10010 -0.494859984 100 61 0 54 0 1.254 80 247 275 332 0 100 208 0 161 0 1.214 80 254 1523 1921 0 100 756 0 548 0 1.2 80 263 6456 7673 0
A#10011 -0.141613979 100 61 0 52 0 1.355 80 229 132 142 0 100 206 0 154 0 1.301 80 230 724 813 0 100 684 0 525 0 1.32 80 230 3254 3397 0
A#10012 100 60 0 52 0 1.77 80 243 45 55 0 100 187 0 149 0 1.569 80 246 270 313 0 100 680 0 518 0 1.734 80 229 1183 1200 0
A#10013 110 60 0 52 0 1.61 80 236 72 83 0 110 178 0 149 0 1.566 80 245 374 440 0 100 652 0 506 0 1.571 80 231 1840 1800 0
A#10014 -0.142604804 100 60 0 50 0 1.524 80 208 82 91 0 100 176 0 141 0 1.53 80 222 459 523 0 100 652 0 459 0 1.421 80 222 2002 2245 0

Total number of rows: 43200

Table truncated, full table size 5561 Kbytes.




Supplementary data files not provided

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