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Sample GSM13278 Query DataSets for GSM13278
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 1
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_004
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.394474942 100 46 0 55 0 2.038 80 390 50 75 0 100 105 0 156 0 1.689 80 390 204 358 0 100 313 0 382 0 1.6 80 390 689 1029 0
A#10 -0.278264753 110 46 0 45 0 1.416 80 355 81 83 0 110 100 0 96 0 1.541 80 355 330 394 0 110 307 0 233 0 1.449 80 338 1317 1126 0
A#100 0.005196296 80 50 0 48 0 2.369 52 197 13 40 0 90 133 0 115 0 2.866 52 263 26 181 0 90 328 0 256 0 1.939 52 214 228 579 0
A#1000 100 67 0 54 0 2.82 80 263 11 19 -50 100 169 0 136 0 3.522 80 244 38 73 -50 100 592 0 326 0 3.545 80 242 116 211 -50
A#10000 -0.083602722 100 60 0 51 0 1.376 80 230 137 125 0 100 162 0 127 0 1.463 80 230 509 570 0 100 463 0 277 0 1.333 80 221 2318 1518 0
A#10001 0.241396163 100 61 0 52 0 1.512 80 213 76 93 0 100 153 0 133 0 1.485 80 227 317 438 0 100 522 0 299 0 1.493 80 216 1395 1121 0
A#10002 -0.060977414 110 63 0 54 0 1.337 80 214 195 171 0 110 152 0 136 0 1.307 80 220 801 856 0 110 533 0 310 0 1.251 80 211 3267 2192 0
A#10003 0.030809675 100 63 0 55 0 1.279 80 204 205 195 0 110 162 0 145 0 1.309 80 239 796 924 0 110 525 0 311 0 1.309 80 204 3765 2481 0
A#10004 0.155788537 110 66 0 58 0 1.174 80 227 884 853 0 110 168 0 150 0 1.197 80 243 3394 3881 0 110 571 0 350 0 1.259 80 236 15371 10240 0
A#10005 0.617910872 110 66 0 59 0 1.553 80 236 102 146 0 110 177 0 154 0 1.42 80 230 428 686 0 100 574 0 357 0 1.301 80 229 1857 1896 0
A#10006 -0.790289576 100 66 0 57 0 1.174 80 251 1088 600 0 100 173 0 154 0 1.185 80 247 4471 2892 0 110 600 0 333 0 1.163 80 220 19567 7579 0
A#10007 0.215146566 110 65 0 56 0 1.218 80 249 712 722 0 110 171 0 147 0 1.194 80 244 3057 3448 0 110 585 0 325 0 1.244 80 218 12651 9017 0
A#10008 0.180374096 110 64 0 56 0 1.264 80 245 292 305 0 110 167 0 142 0 1.234 80 226 1230 1409 0 110 526 0 329 0 1.215 80 208 5039 3811 0
A#10009 0.277500527 90 66 0 56 0 1.567 52 233 71 87 0 90 172 0 151 0 1.492 52 218 303 424 0 90 550 0 328 0 1.508 52 187 1438 1190 0
A#1001 0.160002023 90 61 0 50 0 1.843 52 242 37 59 0 110 145 0 122 0 1.949 80 248 158 275 0 100 467 0 258 0 2.219 80 246 725 774 0
A#10010 -0.424811115 110 64 0 55 0 1.639 80 288 412 301 0 110 171 0 146 0 1.574 80 305 1703 1398 0 110 558 0 310 0 1.57 80 256 7295 3768 0
A#10011 0.088697376 110 62 0 53 0 1.43 80 264 202 199 0 110 158 0 133 0 1.396 80 270 825 940 0 110 522 0 299 0 1.265 80 259 3338 2432 0
A#10012 -0.553776999 100 62 0 52 0 1.542 80 216 62 76 0 100 155 0 127 0 1.628 80 243 265 355 0 100 516 0 293 0 1.53 80 238 1164 943 0
A#10013 0.198786338 100 62 0 54 0 1.918 80 235 70 78 0 100 152 0 127 0 1.462 80 243 292 363 0 110 524 0 294 0 1.721 80 230 1091 954 0
A#10014 0.209674238 100 66 0 54 0 1.548 80 257 178 189 0 100 156 0 129 0 1.339 80 247 666 881 0 110 552 0 304 0 1.784 80 238 2869 2091 0

Total number of rows: 43200

Table truncated, full table size 5569 Kbytes.




Supplementary data files not provided

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