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Sample GSM13279 Query DataSets for GSM13279
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 2
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_005
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 70 67 0 189 0 2.777 32 175 9 236 0 60 219 0 774 0 2.788 32 127 10 1177 0 90 895 0 3564 0 2.976 52 336 1427 2637 -50
A#10 -0.059289143 110 44 0 46 0 1.469 80 336 68 91 0 110 92 0 98 0 1.527 80 337 366 424 0 110 470 0 404 0 1.537 80 342 2498 2219 0
A#100 90 45 0 45 0 2.72 52 230 5 28 -50 90 102 0 97 0 2.623 52 231 31 136 -50 90 521 0 392 0 2.872 52 240 89 718 -50
A#1000 90 58 0 53 0 2.5 52 244 17 20 -50 90 162 0 125 0 2.655 52 244 94 116 -50 90 970 0 545 0 2.71 52 254 717 542 -50
A#10000 -0.105374202 100 59 0 49 0 1.488 80 260 85 88 0 100 164 0 109 0 1.639 80 216 359 431 0 100 980 0 415 0 1.585 80 234 3038 2134 0
A#10001 0.379835119 70 62 0 52 0 1.87 32 179 43 58 0 90 161 0 109 0 1.746 52 209 224 337 0 80 968 0 433 0 1.764 52 200 1519 1689 0
A#10002 0.03884081 110 60 0 51 0 1.517 80 269 104 121 0 110 174 0 117 0 1.476 80 271 533 589 0 100 991 0 461 0 1.349 80 271 3784 2971 0
A#10003 0.485837617 100 60 0 49 0 1.513 80 218 88 139 0 100 171 0 114 0 1.485 80 252 426 681 0 100 960 0 466 0 1.436 80 255 3449 3384 0
A#10004 0.399777223 110 60 0 50 0 1.257 80 224 419 578 0 110 175 0 116 0 1.265 80 249 2123 2794 0 110 941 0 473 0 1.19 80 266 15790 13563 0
A#10005 0.897466706 110 61 0 51 0 1.731 80 238 53 105 0 110 177 0 116 0 1.873 80 238 292 487 0 90 980 0 464 0 1.533 52 232 2106 2552 0
A#10006 -0.710895671 100 62 0 51 0 1.245 80 257 762 480 0 100 187 0 116 0 1.286 80 256 3650 2341 0 100 1021 0 469 0 1.275 80 256 26682 11543 0
A#10007 0.387804932 110 61 0 51 0 1.232 80 236 441 590 0 110 184 0 115 0 1.198 80 235 2133 2862 0 110 1008 0 469 0 1.768 80 238 16351 13833 0
A#10008 0.109794182 110 58 0 50 0 1.258 80 232 203 243 0 100 159 0 114 0 1.268 80 215 1072 1192 0 110 931 0 454 0 1.255 80 230 6946 5719 0
A#10009 0.257842076 80 58 0 50 0 1.869 52 200 44 66 0 90 169 0 116 0 1.998 52 254 196 323 -50 90 993 0 459 0 1.672 52 244 1481 1593 -50
A#1001 0.209913111 100 50 0 46 0 1.755 80 261 69 88 0 100 128 0 97 0 1.646 80 315 305 444 0 100 665 0 371 0 1.57 80 275 2507 2215 0
A#10010 -0.370147437 110 64 0 50 0 1.33 80 301 261 226 0 110 205 0 120 0 1.388 80 326 1321 1102 0 110 1123 0 459 0 1.331 80 326 9035 5364 0
A#10011 0.382249402 110 71 0 52 0 1.494 80 282 110 159 0 110 243 0 124 0 1.473 80 287 573 776 0 110 1276 0 482 0 1.576 80 287 3901 3827 0
A#10012 90 76 0 54 0 2.802 52 221 32 64 -50 90 270 0 127 0 2.604 52 220 105 309 -50 90 1441 0 511 0 2.312 52 235 1238 1543 -50
A#10013 0.274556335 110 65 0 49 0 1.767 80 267 79 106 0 110 214 0 111 0 1.771 80 266 368 548 0 100 1082 5 434 5 1.939 80 264 2921 2583 0
A#10014 0.112234684 110 59 0 47 0 1.619 80 264 77 103 0 90 187 0 103 0 1.556 52 253 401 514 0 100 902 0 390 0 1.652 80 258 3083 2338 0

Total number of rows: 43200

Table truncated, full table size 5544 Kbytes.




Supplementary data files not provided

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