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Sample GSM13280 Query DataSets for GSM13280
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 3
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_006
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.741009144 90 59 0 168 0 1.564 52 313 129 230 0 90 147 0 696 0 1.577 52 312 560 1131 0 100 793 0 3393 0 1.678 80 388 2907 5794 0
A#10 -0.919271526 110 57 0 192 0 1.351 80 330 252 475 0 110 139 0 800 0 1.317 80 352 1024 2320 0 110 771 0 3988 0 1.355 80 373 6800 11650 0
A#100 100 61 0 137 0 3.596 80 226 26 -29 -50 100 172 0 529 0 4.139 80 226 81 -134 -50 100 898 0 2522 0 4.353 80 240 474 -491 -50
A#1000 100 82 0 54 0 2.848 80 219 71 13 -50 100 277 0 124 0 2.699 80 226 509 87 -50 100 1268 0 570 0 3.211 80 223 2267 533 -50
A#10000 -0.020818625 110 63 0 48 0 1.353 80 202 158 151 0 110 175 0 101 0 1.421 80 197 614 742 0 110 987 0 391 0 1.389 80 200 4305 3684 0
A#10001 0.020654906 110 63 0 48 0 1.456 80 168 115 115 0 110 172 0 101 0 1.615 80 159 497 574 0 100 942 0 391 0 1.372 80 162 3303 2823 0
A#10002 0.006931673 110 65 0 48 0 1.29 80 178 246 217 0 110 184 0 106 0 1.281 80 175 1030 1060 0 110 972 0 414 0 1.304 80 182 6520 5363 0
A#10003 -0.511467452 110 67 0 50 0 1.239 80 216 223 284 0 110 188 0 108 0 1.298 80 227 917 1327 0 110 1007 0 448 0 1.243 80 220 6436 6688 0
A#10004 -0.045977289 110 67 0 48 0 1.107 80 244 945 866 0 110 180 0 106 0 1.114 80 258 3975 4217 0 110 1025 0 443 0 1.12 80 251 27186 21016 0
A#10005 0.136756348 110 66 0 47 0 1.423 80 178 110 108 0 110 168 0 102 0 1.357 80 211 516 510 0 110 957 0 405 0 1.365 80 216 3060 2577 0
A#10006 0.192009666 120 67 0 47 0 2.09 120 149 935 739 0 120 168 0 103 0 1.982 120 162 3853 3503 0 120 947 0 405 0 2.044 120 169 26373 17707 0
A#10007 -0.014433877 110 67 0 47 0 1.255 80 175 770 717 0 110 175 0 104 0 1.22 80 150 3331 3312 0 110 1016 0 415 0 1.232 80 164 22603 16937 0
A#10008 0.16896929 110 65 0 47 0 1.188 80 196 454 365 0 110 169 0 102 0 1.201 80 180 1991 1772 0 110 852 0 395 0 1.171 80 181 12523 8949 0
A#10009 -0.053440967 90 62 0 47 0 1.669 52 196 82 80 0 100 159 0 99 0 1.58 80 198 285 377 0 100 717 0 382 0 1.567 80 187 2069 1891 0
A#1001 -0.488075942 110 76 0 50 0 1.342 80 237 180 251 0 110 232 0 111 0 1.291 80 252 744 1138 0 110 1117 0 487 0 1.325 80 238 4862 6043 0
A#10010 -0.379130771 110 64 0 47 0 1.179 80 264 468 523 0 110 160 0 96 0 1.225 80 245 1951 2518 0 110 801 0 381 0 1.233 80 241 13617 12598 0
A#10011 -0.126391371 110 65 0 46 0 1.258 80 266 253 245 0 110 157 0 94 0 1.306 80 236 1027 1187 0 110 797 0 368 0 1.274 80 252 7005 5917 0
A#10012 -0.252938659 90 63 0 46 0 1.733 52 198 48 60 0 100 156 0 94 0 1.853 80 195 212 291 0 100 802 0 372 0 1.626 80 223 1326 1464 0
A#10013 0.039697979 120 62 0 47 0 1.898 120 175 202 187 0 100 156 0 101 0 1.297 80 193 925 931 0 110 807 0 393 0 1.325 80 187 5667 4595 0
A#10014 0.216383298 110 63 0 47 0 1.386 80 199 226 185 0 110 151 0 100 0 1.348 80 219 1038 901 0 110 842 0 375 0 1.314 80 215 6270 4650 0

Total number of rows: 43200

Table truncated, full table size 5673 Kbytes.




Supplementary data files not provided

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