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Sample GSM13281 Query DataSets for GSM13281
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 4
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_007
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.579501543 100 51 0 68 0 1.539 80 367 67 240 0 100 151 0 216 0 1.709 80 367 397 1162 0 110 850 0 973 0 1.573 80 449 2265 6104 0
A#10 -0.422139749 110 47 0 47 0 1.537 80 349 81 273 0 110 120 0 106 0 1.624 80 349 429 1274 0 120 617 0 453 0 1.973 120 384 3046 6909 0
A#100 -0.724021529 120 49 0 50 0 2.423 120 165 16 205 0 110 150 0 123 0 2.518 80 213 36 983 0 120 721 0 507 0 2.167 120 158 1011 5419 0
A#1000 100 64 0 53 0 2.607 80 236 28 15 -50 100 203 0 127 0 3.07 80 231 142 73 -50 100 1231 0 591 0 3.492 80 242 1000 403 -50
A#10000 -0.149984404 110 64 0 54 0 1.504 80 223 93 156 0 110 203 0 137 0 1.37 80 223 519 756 0 110 1126 0 582 0 1.375 80 215 3747 3907 0
A#10001 -0.09692672 110 63 0 52 0 1.494 80 189 85 131 0 100 201 0 130 0 1.522 80 195 416 614 0 110 1093 0 536 0 1.358 80 210 3072 3182 0
A#10002 -0.350163212 110 63 0 53 0 1.445 80 149 103 204 0 110 215 0 134 0 1.469 80 168 596 938 0 110 1123 0 549 0 1.346 80 166 3563 4714 0
A#10003 -0.06761761 110 67 0 57 0 1.431 80 175 97 151 0 110 227 0 145 0 1.451 80 170 515 709 0 110 1148 0 599 0 1.383 80 172 3905 3643 0
A#10004 -0.11173963 120 67 0 55 0 1.734 120 193 495 614 0 120 240 0 138 0 2.056 120 191 2679 2968 0 120 1137 0 599 0 1.828 120 185 18608 14794 0
A#10005 -0.024010278 110 66 0 53 0 1.518 80 185 121 167 0 100 251 0 138 0 1.644 80 193 685 805 0 100 1216 0 579 0 1.43 80 167 4602 4015 0
A#10006 0.086009429 110 77 0 58 0 1.256 80 244 506 509 0 110 329 0 158 0 1.265 80 263 2755 2447 0 110 1565 0 644 0 1.25 80 231 19371 12214 0
A#10007 -0.082585898 100 94 0 59 0 1.25 80 246 447 538 0 110 413 0 169 0 1.279 80 269 2479 2615 0 110 1907 0 680 0 1.238 80 254 16525 12893 0
A#10008 0.165457405 110 86 0 57 0 1.286 80 197 260 261 0 110 362 0 154 0 1.297 80 216 1435 1213 0 110 1725 0 609 0 1.281 80 215 9029 6089 0
A#10009 100 70 0 53 0 2.284 80 193 52 128 -50 100 246 0 132 0 2.525 80 201 274 581 -50 100 1318 0 524 0 1.739 80 204 2104 3026 -50
A#1001 -0.367102479 110 59 0 51 0 1.46 80 208 150 353 0 110 170 0 115 0 1.452 80 215 711 1613 0 110 971 0 515 0 1.369 80 219 6088 9029 0
A#10010 -0.110471842 110 69 0 53 0 1.206 80 252 281 356 0 110 242 0 132 0 1.279 80 230 1485 1700 0 110 1294 0 534 0 1.237 80 239 10065 8627 0
A#10011 0.050069771 110 69 0 53 0 1.744 80 246 125 161 0 110 261 0 135 0 1.718 80 228 739 768 0 110 1264 0 537 0 1.567 80 234 4732 3893 0
A#10012 0.155155893 90 67 0 53 0 2.214 52 213 37 84 0 100 203 0 126 0 1.968 80 230 222 381 -50 80 1172 0 531 0 1.724 52 189 1651 1995 0
A#10013 0.427441183 110 63 0 53 0 1.314 80 239 211 164 0 110 184 0 122 0 1.345 80 201 1112 776 0 110 1025 0 504 0 1.295 80 283 7545 3882 0
A#10014 0.445942313 100 61 0 52 0 1.341 80 200 202 152 0 110 189 0 123 0 1.379 80 188 1072 751 0 110 968 0 498 0 1.295 80 257 7194 3579 0

Total number of rows: 43200

Table truncated, full table size 5674 Kbytes.




Supplementary data files not provided

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