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Sample GSM13282 Query DataSets for GSM13282
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 5
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_008
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.38115423 110 53 0 41 0 1.63 80 446 128 132 0 110 132 0 80 0 1.697 80 453 544 694 0 110 396 0 273 0 1.431 80 424 3042 3381 0
A#10 -0.349399745 110 52 0 41 0 1.287 80 343 377 376 0 110 120 0 86 0 1.352 80 343 1611 2013 0 110 412 0 295 0 1.234 80 343 8942 9185 0
A#100 -1.413583609 100 58 0 45 0 2.516 80 219 35 42 -50 100 162 0 106 0 2.994 80 236 101 201 -50 100 564 0 409 0 2.514 80 278 734 972 0
A#1000 100 85 0 59 0 3.074 80 211 -3 -2 -50 100 244 0 164 0 2.584 80 208 5 -5 -50 100 986 0 628 0 3.275 80 229 85 -1 -50
A#10000 -0.347675655 110 73 0 49 0 1.446 80 225 194 181 0 100 197 0 115 0 1.411 80 222 858 984 0 110 702 0 387 0 1.282 80 207 4144 4202 0
A#10001 -0.175747291 110 71 0 48 0 1.479 80 200 193 166 0 110 196 0 112 0 1.501 80 200 897 888 0 110 695 0 385 0 1.407 80 210 3905 3878 0
A#10002 -0.163787925 110 71 0 49 0 1.386 80 170 278 222 0 110 209 0 114 0 1.314 80 184 1194 1185 0 110 784 0 420 0 1.244 80 198 5321 5163 0
A#10003 -0.354197733 110 76 0 51 0 1.38 80 207 325 279 0 110 210 0 119 0 1.324 80 209 1337 1482 0 110 781 0 441 0 1.258 80 202 6077 6603 0
A#10004 0.057281738 110 75 0 52 0 1.224 80 243 671 510 0 110 209 0 128 0 1.248 80 229 2896 2755 0 110 744 0 447 0 1.226 80 228 14165 11963 0
A#10005 0.14746643 110 72 0 51 0 1.318 80 211 285 208 0 110 216 0 128 0 1.294 80 211 1264 1130 0 110 757 0 449 0 1.254 80 218 6215 4786 0
A#10006 -0.394124875 120 75 0 51 0 1.878 120 180 628 566 0 120 231 0 128 0 1.907 120 183 2477 3002 0 120 727 0 468 0 1.91 120 180 12871 13556 0
A#10007 -0.370510657 110 76 0 51 0 1.183 80 161 782 682 0 110 227 0 125 0 1.175 80 185 3215 3653 0 110 660 0 463 0 1.152 80 167 15616 16094 0
A#10008 0.005227066 120 70 0 50 0 1.725 120 140 378 293 0 110 217 0 121 0 1.29 80 186 1694 1613 0 110 675 0 434 0 1.267 80 168 8313 6966 0
A#10009 0.069014541 110 73 0 51 0 1.564 80 168 191 144 0 110 206 0 122 0 1.501 80 205 801 756 0 110 729 0 430 0 1.431 80 201 3614 3218 0
A#1001 0.531706556 110 69 0 53 0 1.32 80 225 305 223 0 110 211 0 133 0 1.318 80 225 1191 1197 0 110 753 0 511 0 1.227 80 239 7570 5401 0
A#10010 -0.696891557 110 74 0 50 0 1.316 80 246 378 392 0 110 205 0 120 0 1.313 80 251 1572 2039 0 110 729 0 421 0 1.226 80 254 7510 8895 0
A#10011 -0.185714299 110 74 0 50 0 1.47 80 269 212 170 0 110 209 0 116 0 1.439 80 221 817 884 0 110 693 0 410 0 1.382 80 240 4061 4085 0
A#10012 -0.428470317 90 72 0 49 0 2.027 52 198 83 81 0 110 203 0 113 0 2.028 80 194 354 415 0 100 625 0 410 0 1.706 80 196 1605 1850 0
A#10013 -0.094696009 110 70 0 49 0 1.273 80 211 377 299 0 110 203 0 110 0 1.36 80 208 1618 1630 0 110 648 0 413 0 1.295 80 208 7858 7010 0
A#10014 -0.218329191 110 70 0 49 0 1.3 80 234 342 278 0 110 202 0 109 0 1.353 80 226 1346 1474 0 110 717 0 385 0 1.326 80 231 6638 6440 0

Total number of rows: 43200

Table truncated, full table size 5697 Kbytes.




Supplementary data files not provided
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