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Sample GSM13283 Query DataSets for GSM13283
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 6
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_009
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.396092047 110 35 0 44 0 1.801 80 469 34 92 0 110 60 0 72 0 1.623 80 473 191 212 0 110 408 0 1020 0 1.432 80 467 2794 4977 0
A#10 0.023689718 110 35 0 58 0 1.532 80 331 68 125 0 110 59 0 104 0 1.404 80 317 346 299 0 110 444 0 1754 0 1.307 80 315 5251 7075 0
A#100 100 36 0 39 0 3.613 80 265 5 15 -50 100 65 0 56 0 3.091 80 203 31 38 -50 60 601 0 960 0 1.826 32 119 386 709 0
A#1000 100 38 0 35 0 3.618 80 244 4 6 -50 100 65 0 48 0 3.561 80 235 31 11 -50 100 610 0 400 0 4.359 80 221 184 353 -100
A#10000 -0.229101889 100 48 0 37 0 1.503 80 235 112 62 0 90 111 0 54 0 1.677 52 235 424 140 0 100 930 0 484 0 1.417 80 222 5951 3419 0
A#10001 0.055396636 110 45 0 37 0 1.612 80 187 75 54 0 90 108 0 55 0 1.628 52 199 294 121 0 110 930 0 488 0 1.438 80 208 4357 3067 0
A#10002 -0.060141488 110 48 0 39 0 1.349 80 166 162 97 0 110 111 0 57 0 1.963 80 172 654 224 0 110 1084 0 581 0 1.276 80 199 9372 5477 0
A#10003 -0.101607809 110 50 0 39 0 1.427 80 216 201 117 0 110 110 0 56 0 1.427 80 179 792 269 0 110 1106 0 565 0 1.204 80 215 11007 6523 0
A#10004 0.054034927 110 50 0 38 0 1.201 80 249 429 282 0 120 112 0 57 0 2.655 120 223 1723 613 0 110 1083 0 548 0 1.172 80 236 24831 15310 0
A#10005 0.190762893 110 49 0 38 0 1.447 80 236 105 91 0 100 108 0 55 0 1.896 80 174 460 196 0 110 1023 0 613 0 1.29 80 240 6806 5108 0
A#10006 -0.577324772 110 50 0 38 0 1.652 80 218 871 336 0 120 101 0 56 0 2.368 120 155 3282 762 0 110 1008 2 586 0 1.426 80 201 48704 18358 0
A#10007 0.206567276 110 50 0 38 0 1.196 80 191 554 387 0 120 111 0 57 0 2.748 120 174 2279 883 0 110 1094 0 569 0 1.292 80 183 33936 21522 0
A#10008 -0.185602876 120 50 0 38 0 1.685 120 139 320 184 0 110 111 0 57 0 1.214 80 174 1391 428 0 110 1010 0 561 0 1.155 80 184 19929 10532 0
A#10009 0.095149624 100 49 0 38 0 1.842 80 171 43 31 0 100 102 0 56 0 1.987 80 192 172 77 -50 90 956 0 545 0 1.603 52 192 2288 1903 0
A#1001 -0.007527057 110 38 0 34 0 2.647 80 257 14 39 0 110 68 0 46 0 1.981 80 254 63 89 0 120 614 0 342 0 2.009 120 208 1225 2093 0
A#10010 -0.512770202 110 49 0 38 0 1.256 80 277 451 201 0 110 96 0 55 0 1.546 80 238 1786 442 0 110 964 0 509 0 1.283 80 283 26277 11008 0
A#10011 -0.53209148 100 47 0 37 0 1.727 80 238 80 43 0 100 94 0 52 0 2.264 80 262 294 88 0 110 844 0 451 0 1.766 80 241 3927 2366 0
A#10012 0.083693361 100 45 0 36 0 1.558 80 190 75 55 0 100 91 0 52 0 1.963 80 238 291 124 0 110 830 0 417 0 1.432 80 186 4161 2985 0
A#10013 -0.136270677 110 48 0 37 0 1.353 80 223 167 95 0 110 101 0 53 0 1.601 80 236 640 219 0 110 907 0 479 0 1.307 80 208 9349 5116 0
A#10014 -0.045210553 110 53 0 39 0 1.355 80 255 240 147 0 110 113 0 56 0 1.553 80 249 997 348 0 110 1160 0 572 0 1.35 80 242 14769 8943 0

Total number of rows: 43200

Table truncated, full table size 5555 Kbytes.




Supplementary data files not provided

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