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Sample GSM13284 Query DataSets for GSM13284
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 7
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 24h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_010
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 110 37 0 37 0 3.404 80 455 15 21 0 110 68 0 61 0 2.143 80 449 59 103 0 110 157 0 157 0 2.65 80 457 302 391 0
A#10 -0.198089205 110 39 0 37 0 1.246 80 349 205 138 0 110 66 0 61 0 1.239 80 365 738 607 0 110 167 0 163 0 1.221 80 364 3534 2222 0
A#100 0.80211109 90 41 0 37 0 2.215 52 233 24 24 0 100 72 0 62 0 3.023 80 239 79 103 0 100 193 0 163 0 1.892 80 257 329 449 0
A#1000 100 45 0 43 0 3.315 80 242 3 2 -50 100 82 0 78 0 3.781 80 245 20 18 -50 100 271 0 209 0 3.562 80 238 -6 98 -50
A#10000 0.135354768 110 50 0 45 0 1.338 80 217 153 117 0 110 107 0 87 0 1.356 80 221 595 580 0 110 336 0 245 0 1.28 80 230 2756 1979 0
A#10001 0.401424935 110 52 0 45 0 1.383 80 234 131 116 0 110 110 0 87 0 1.352 80 209 482 516 0 100 361 0 244 0 1.339 80 227 2328 1885 0
A#10002 0.045353423 110 53 0 47 0 1.311 80 216 207 155 0 100 117 0 95 0 1.278 80 212 798 736 0 110 384 0 261 0 1.591 80 220 3658 2463 0
A#10003 0.378663056 120 52 0 47 0 1.988 120 153 216 186 0 110 121 0 94 0 1.254 80 220 806 879 0 110 387 0 256 0 1.208 80 192 3974 3138 0
A#10004 0.123057033 120 49 0 44 0 2.087 120 144 525 415 0 120 109 0 87 0 1.94 120 207 1966 1870 0 120 341 0 242 0 1.89 120 171 9171 6730 0
A#10005 0.585048544 110 50 0 46 0 1.415 80 191 119 107 0 110 109 0 88 0 1.415 80 207 446 536 0 110 342 0 240 0 1.354 80 191 1994 1813 0
A#10006 -0.266152708 110 51 0 46 0 1.232 80 223 697 459 0 110 111 0 91 0 1.246 80 227 2514 2141 0 110 342 0 239 0 1.222 80 221 12752 7446 0
A#10007 0.141505088 120 50 0 45 0 1.926 120 206 437 347 0 120 103 0 87 0 2.275 120 211 1629 1600 0 120 340 0 246 0 2 120 215 7821 5662 0
A#10008 0.211200149 110 49 0 45 0 1.308 80 190 254 206 0 110 98 0 87 0 1.241 80 191 990 942 0 110 330 0 246 0 1.256 80 199 4429 3370 0
A#10009 0.255274245 100 48 0 44 0 1.353 80 215 94 80 0 100 98 0 84 0 1.35 80 215 348 360 0 100 292 0 229 0 1.32 80 202 1734 1284 0
A#1001 0.317976294 110 43 0 41 0 1.252 80 231 195 171 0 110 80 0 72 0 1.25 80 234 710 769 0 100 246 0 198 0 1.25 80 231 3565 2824 0
A#10010 -0.002275685 110 47 0 43 0 1.323 80 251 352 259 0 110 93 0 77 0 1.28 80 251 1232 1111 0 120 265 0 210 0 1.918 120 210 5133 3454 0
A#10011 0.203290821 110 47 0 43 0 1.242 80 251 187 145 0 110 94 0 76 0 1.274 80 251 670 653 0 110 269 0 191 0 1.219 80 212 3139 2406 0
A#10012 0.356787864 110 47 0 43 0 1.511 80 211 63 58 0 100 91 0 79 0 1.454 80 205 241 256 0 110 276 0 202 0 1.447 80 197 1215 971 0
A#10013 0.219372796 100 48 0 44 0 1.384 80 184 204 167 0 110 93 0 84 0 1.406 80 191 747 741 0 100 293 0 225 0 1.354 80 178 3753 2693 0
A#10014 0.111826361 110 49 0 44 0 1.257 80 200 216 163 0 110 97 0 86 0 1.283 80 224 809 724 0 110 295 0 224 0 1.219 80 200 3522 2646 0

Total number of rows: 43200

Table truncated, full table size 5582 Kbytes.




Supplementary data files not provided

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