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Sample GSM13286 Query DataSets for GSM13286
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 17
Sample type RNA
 
Channel 1
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 6 animals (No. N1, N2, N3, N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool3 starved = 6 animals (No. St1, St2, St3, St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_012
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.070368415 130 48 0 45 0 2.677 120 609 28 55 0 110 81 0 61 0 2.668 80 453 103 123 0 110 435 0 433 0 2.3 80 454 758 1464 0
A#10 -0.138258545 110 50 0 47 0 1.34 80 329 242 340 0 110 94 0 65 0 1.323 80 329 729 741 0 110 511 0 477 0 1.277 80 343 6218 9089 0
A#100 0.365664933 100 58 0 57 0 1.982 80 280 76 90 0 100 122 0 84 0 1.678 80 260 219 184 0 100 660 0 706 0 1.679 80 269 1857 2347 0
A#1000 100 86 0 81 0 2.127 80 223 46 43 -50 100 201 0 130 0 2.439 80 224 136 100 -50 100 1364 0 1156 0 2.324 80 222 1093 1121 -50
A#10000 -0.510910375 100 63 0 59 0 1.336 80 217 129 225 0 110 139 0 88 0 1.471 80 229 349 479 0 100 858 0 698 0 1.358 80 241 2992 5629 0
A#10001 -0.085239912 100 64 0 56 0 1.584 80 247 96 155 0 100 137 0 85 0 1.706 80 226 324 328 0 100 850 0 669 0 1.485 80 260 2487 3799 0
A#10002 0.049948986 110 68 0 61 0 1.294 80 236 215 277 0 100 145 0 89 0 1.34 80 208 625 594 0 110 985 0 755 0 1.339 80 257 4983 6937 0
A#10003 0.0764441 110 72 0 63 0 1.385 80 250 253 355 0 110 153 0 97 0 1.341 80 235 822 741 0 110 962 0 793 0 1.294 80 250 6610 8793 0
A#10004 0.095874203 110 70 0 63 0 1.231 80 252 653 805 0 110 151 0 96 0 1.281 80 245 2035 1718 0 110 924 0 780 0 1.231 80 245 16750 19818 0
A#10005 -0.157482123 100 69 0 62 0 1.479 80 217 119 203 0 100 148 0 93 0 1.394 80 217 396 421 0 100 895 0 770 0 1.321 80 217 3216 4765 0
A#10006 0.196512338 100 67 0 60 0 1.175 80 221 712 829 0 100 142 0 92 0 1.177 80 233 2172 1715 0 100 781 0 721 0 1.175 80 218 17986 20616 0
A#10007 0.01863329 110 68 0 61 0 1.189 80 238 572 734 0 110 145 0 92 0 1.328 80 244 1647 1521 0 110 876 0 730 0 1.203 80 244 14403 18042 0
A#10008 -0.030891333 100 72 0 63 0 1.227 80 238 295 430 0 110 141 0 95 0 1.221 80 231 888 894 0 100 915 0 753 0 1.223 80 258 7337 10534 0
A#10009 90 73 0 66 0 2.216 52 272 43 82 -50 90 140 0 97 0 2.192 52 240 167 173 -50 60 1003 0 832 0 1.606 32 159 1650 2213 0
A#1001 0.288253807 110 77 0 71 0 1.375 80 240 196 238 0 110 168 0 116 0 1.318 80 237 594 506 0 110 1103 0 1019 0 1.339 80 240 4832 5946 0
A#10010 -0.518750679 110 68 0 64 0 1.342 80 370 362 616 0 110 140 0 92 0 1.411 80 312 1099 1336 0 110 856 0 741 0 1.318 80 334 8991 15533 0
A#10011 -0.177229772 100 63 0 57 0 1.367 80 251 183 272 0 100 128 0 87 0 1.314 80 266 527 545 0 100 796 0 694 0 1.332 80 266 3952 6283 0
A#10012 0.213672349 100 64 0 58 0 1.729 80 245 75 103 0 90 126 0 84 0 1.826 52 247 266 238 0 90 744 0 694 0 1.67 52 248 1914 2678 0
A#10013 0.081628104 100 64 0 60 0 1.382 80 225 148 212 0 100 130 0 85 0 1.51 80 240 457 460 0 100 719 0 694 0 1.427 80 237 3917 5262 0
A#10014 0.211247352 100 66 0 61 0 1.31 80 236 156 198 0 100 136 0 89 0 1.516 80 239 451 446 0 100 791 0 710 0 1.314 80 239 3927 5055 0

Total number of rows: 43200

Table truncated, full table size 5604 Kbytes.




Supplementary data files not provided

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