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Sample GSM13288 Query DataSets for GSM13288
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 19
Sample type RNA
 
Channel 1
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 6 animals (No. N1, N2, N3, N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool3 starved = 6 animals (No. St1, St2, St3, St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_014
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.094573976 100 39 0 44 0 1.71 80 306 48 90 0 100 74 0 90 0 1.625 80 306 213 406 0 100 300 0 429 0 1.552 80 307 1313 2465 0
A#10 -0.341958766 100 38 0 42 0 1.225 80 276 267 316 0 100 72 0 77 0 1.233 80 281 1050 1330 0 100 285 0 355 0 1.2 80 276 6892 7994 0
A#100 -0.43492936 100 46 0 49 0 1.549 80 253 47 69 0 100 106 0 113 0 1.978 80 301 176 292 0 100 511 0 551 0 1.597 80 296 1319 1753 0
A#1000 90 58 0 58 0 3 52 221 9 5 -50 90 162 0 142 0 2.875 52 221 11 6 -50 90 774 0 765 0 4.204 52 232 205 5 -50
A#10000 -0.322860667 100 44 0 46 0 1.481 80 235 83 101 0 100 104 0 96 0 1.435 80 230 347 444 0 100 410 0 437 0 1.442 80 223 2236 2473 0
A#10001 -1.829152868 100 45 0 47 0 1.547 80 255 72 76 0 100 104 0 97 0 1.529 80 236 311 307 0 100 432 0 445 0 1.464 80 244 1920 1679 0
A#10002 0.297267267 100 45 0 47 0 1.314 80 231 128 111 0 100 107 0 100 0 1.277 80 225 565 484 0 100 428 0 482 0 1.35 80 202 3355 2896 0
A#10003 0.123097108 100 44 0 48 0 1.24 80 222 174 166 0 100 106 0 101 0 1.333 80 198 707 711 0 100 404 0 466 0 1.316 80 203 4548 3860 0
A#10004 0.528120411 100 44 0 47 0 1.158 80 223 462 336 0 100 95 0 103 0 1.21 80 211 2012 1414 0 100 414 0 451 0 1.17 80 239 12182 8413 0
A#10005 0.176618113 100 44 0 46 0 1.491 80 225 85 81 0 100 98 0 104 0 1.477 80 231 366 343 0 100 410 0 444 0 1.396 80 231 2134 1984 0
A#10006 0.074172043 110 46 0 46 0 1.185 80 230 407 383 0 110 102 0 103 0 1.21 80 217 1815 1656 0 110 429 0 444 0 1.171 80 231 10906 9108 0
A#10007 0.396558667 110 47 0 47 0 1.305 80 238 288 232 0 110 99 0 98 0 1.251 80 236 1269 982 0 110 457 0 442 0 1.276 80 245 7559 5642 0
A#10008 -0.240144949 100 47 0 47 0 1.262 80 229 190 220 0 100 97 0 98 0 1.263 80 251 904 965 0 100 432 0 443 0 1.243 80 246 5074 5563 0
A#10009 0.100979068 100 46 0 47 0 1.944 80 228 49 51 0 100 95 0 98 0 1.79 80 234 206 225 0 90 385 0 425 0 1.575 52 221 1525 1251 0
A#1001 -0.04163224 100 53 0 53 0 1.236 80 240 234 237 0 100 128 0 121 0 1.29 80 242 854 947 0 100 649 0 627 0 1.244 80 234 5932 5609 0
A#10010 -0.583056467 110 45 0 46 0 1.263 80 248 203 284 0 110 93 0 98 0 1.27 80 258 933 1154 0 110 412 0 428 0 1.245 80 290 5483 6848 0
A#10011 -0.32056437 100 45 0 47 0 1.487 80 269 86 107 0 100 101 0 100 0 1.484 80 276 371 444 0 100 421 0 435 0 1.449 80 264 2280 2624 0
A#10012 -0.230339869 90 46 0 47 0 2.203 52 245 31 44 0 90 104 0 100 0 1.965 52 257 163 168 0 90 443 0 436 0 2.258 52 256 829 931 0
A#10013 -0.039549775 100 45 0 48 0 1.418 80 247 103 113 0 100 103 0 105 0 1.395 80 254 470 484 0 100 458 0 454 0 1.364 80 263 2782 2690 0
A#10014 -0.093302223 100 45 0 48 0 1.495 80 222 77 86 0 100 97 0 97 0 1.483 80 213 353 387 0 100 429 0 434 0 1.402 80 220 2075 2139 0

Total number of rows: 43200

Table truncated, full table size 5532 Kbytes.




Supplementary data files not provided

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