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Sample GSM13291 Query DataSets for GSM13291
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 12
Sample type RNA
 
Channel 1
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_017
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.038071463 100 43 0 38 0 1.524 80 322 85 87 0 100 76 0 79 0 1.458 80 321 333 487 0 100 244 0 260 0 1.483 80 307 1709 2088 0
A#10 -0.113980142 100 43 0 38 0 1.32 80 275 183 190 0 110 73 0 80 0 1.295 80 286 739 1001 0 110 239 0 247 0 1.273 80 292 3897 4543 0
A#100 80 45 0 40 0 2.712 52 199 11 23 0 90 84 0 81 0 2.631 52 233 25 135 0 100 270 0 276 0 2.955 80 247 136 597 0
A#1000 90 61 0 49 0 2.899 52 221 30 11 -50 90 145 0 125 0 2.453 52 209 103 62 -50 90 503 0 465 0 2.791 52 221 524 242 -50
A#10000 110 59 0 47 0 1.437 80 208 118 144 0 110 131 0 118 0 1.36 80 215 483 799 0 110 454 0 423 0 1.341 80 215 2213 3328 0
A#10001 100 58 0 47 0 1.58 80 211 80 90 0 100 136 0 114 0 1.569 80 211 338 477 0 100 507 0 431 0 1.345 80 211 1738 2207 0
A#10002 0.306230526 110 59 0 49 0 1.328 80 223 191 143 0 100 145 0 125 0 1.329 80 218 695 780 0 100 594 0 466 0 1.288 80 231 3453 3540 0
A#10003 0.096873322 110 63 0 51 0 1.401 80 215 153 143 0 110 160 0 137 0 1.337 80 201 572 795 0 110 604 0 499 0 1.367 80 214 3125 3249 0
A#10004 -0.01464382 100 65 0 52 0 1.14 80 206 767 634 0 100 164 0 136 0 1.166 80 207 2895 3427 0 100 588 0 511 0 1.134 80 207 13904 14863 0
A#10005 -0.318308074 110 63 0 50 0 1.511 80 209 123 148 0 100 158 0 127 0 1.398 80 216 489 772 0 100 578 0 460 0 1.336 80 210 2287 3389 0
A#10006 0.901310472 100 63 0 51 0 1.157 80 222 743 387 0 100 154 0 123 0 1.158 80 204 3106 2072 0 100 603 0 431 0 1.145 80 205 14855 9128 0
A#10007 -0.102825447 110 62 0 50 0 1.193 80 233 504 464 0 110 154 0 118 0 1.178 80 221 2020 2570 0 110 562 0 442 0 1.179 80 228 9803 11290 0
A#10008 -0.090672833 110 60 0 50 0 1.23 80 241 270 255 0 110 151 0 126 0 1.235 80 267 1088 1451 0 110 562 0 458 0 1.215 80 260 5117 6346 0
A#10009 60 68 0 55 0 2.009 32 131 66 56 0 60 161 0 159 0 1.584 32 137 211 309 0 60 693 0 606 0 1.467 32 137 861 1459 0
A#1001 0.180283954 110 54 0 46 0 1.355 80 257 140 125 0 110 121 0 109 0 1.384 80 258 535 696 0 100 443 0 378 0 1.342 80 259 2999 3122 0
A#10010 -0.506497592 100 61 0 50 0 1.179 80 313 317 404 0 100 139 0 127 0 1.182 80 282 1319 2171 0 100 517 0 454 0 1.21 80 270 6583 9761 0
A#10011 -0.035048434 100 58 0 46 0 1.238 80 248 170 167 0 100 132 0 116 0 1.283 80 245 670 864 0 100 468 0 383 0 1.265 80 228 3069 3910 0
A#10012 100 56 0 47 0 1.731 80 242 63 62 0 90 139 0 112 0 1.529 52 221 286 358 0 90 522 0 374 0 1.544 52 217 1252 1615 0
A#10013 110 57 0 47 0 1.566 80 244 136 111 0 110 133 0 112 0 1.433 80 263 492 607 0 110 528 0 380 0 1.491 80 266 2609 2905 0
A#10014 -2.190864285 110 58 0 48 0 1.369 80 241 150 134 0 110 131 0 115 0 1.317 80 247 593 742 0 110 491 0 391 0 1.346 80 251 2881 3195 0

Total number of rows: 43200

Table truncated, full table size 5477 Kbytes.




Supplementary data files not provided

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