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Sample GSM13293 Query DataSets for GSM13293
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 14
Sample type RNA
 
Channel 1
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_019
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.057979566 100 46 0 45 0 1.602 80 289 91 100 0 100 93 0 100 0 1.501 80 293 339 421 0 100 461 0 495 0 1.506 80 292 2078 2336 0
A#10 -0.21794082 120 49 0 49 0 1.647 120 298 103 118 0 120 95 0 114 0 2.035 120 298 394 538 0 120 517 0 589 0 1.82 120 319 2488 3122 0
A#100 100 57 0 51 0 1.928 80 297 53 52 0 100 119 0 118 0 2.09 80 266 198 240 0 100 677 0 585 0 1.831 80 275 1520 1441 0
A#1000 100 49 0 49 0 3.543 80 215 6 3 -50 100 98 0 107 0 3.676 80 199 41 17 -50 100 496 0 515 0 3.342 80 197 319 76 -50
A#10000 110 63 0 60 0 1.52 80 227 119 145 0 110 148 0 161 0 1.653 80 227 417 594 0 110 851 0 801 0 1.577 80 246 3201 3552 0
A#10001 100 63 0 57 0 2.426 80 238 18 53 -50 100 139 0 153 0 2.534 80 237 46 213 -50 100 794 0 727 0 2.577 80 241 455 1324 -50
A#10002 0.309511626 100 64 0 59 0 1.339 80 222 199 180 0 100 142 0 157 0 1.275 80 237 631 783 0 100 814 0 728 0 1.32 80 222 5241 4630 0
A#10003 0.149315391 110 66 0 60 0 1.243 80 208 279 288 0 110 152 0 156 0 1.272 80 240 921 1212 0 110 824 0 750 0 1.231 80 227 7092 6815 0
A#10004 0.201595711 100 65 0 59 0 1.192 80 199 549 491 0 100 154 0 162 0 1.179 80 251 1753 2117 0 100 827 0 787 0 1.165 80 228 13931 12441 0
A#10005 0.14902257 110 70 0 64 0 1.522 80 242 126 136 0 110 154 0 172 0 1.456 80 262 430 603 0 110 858 0 840 0 1.383 80 239 3363 3248 0
A#10006 0.804900086 110 67 0 63 0 1.153 80 249 554 353 0 110 146 0 159 0 1.182 80 221 1803 1532 0 110 815 0 778 0 1.184 80 243 13273 8534 0
A#10007 0.167855657 100 64 0 58 0 1.198 80 225 433 408 0 100 140 0 148 0 1.189 80 212 1397 1702 0 100 793 0 693 0 1.209 80 224 10556 9853 0
A#10008 -0.083369771 110 63 0 58 0 1.31 80 213 236 273 0 110 149 0 150 0 1.31 80 210 727 1101 0 110 790 0 696 0 1.247 80 205 5879 6718 0
A#10009 100 60 0 56 0 3.236 80 199 6 28 -50 100 144 0 145 0 3.948 80 213 13 108 -50 100 812 0 695 0 3.283 80 199 3 809 -50
A#1001 0.446178068 110 47 0 48 0 1.903 80 259 103 86 0 110 87 0 101 0 1.65 80 217 386 373 0 110 437 0 490 0 1.607 80 205 2564 1978 0
A#10010 -0.229467295 100 63 0 57 0 1.234 80 265 228 291 0 100 144 0 143 0 1.257 80 242 745 1244 0 100 833 0 694 0 1.264 80 235 6122 7404 0
A#10011 0.012722028 100 69 0 60 0 1.41 80 277 135 154 0 110 154 0 153 0 1.465 80 255 456 682 0 100 907 0 744 0 1.303 80 258 3689 3850 0
A#10012 80 64 0 60 0 1.66 52 210 91 89 0 80 149 0 148 0 1.707 52 209 311 391 0 80 820 0 757 0 1.486 52 199 2000 2091 0
A#10013 100 62 0 59 0 1.356 80 219 172 174 0 100 136 0 149 0 1.374 80 234 593 760 0 100 763 0 741 0 1.379 80 234 4292 4544 0
A#10014 0.238424648 100 65 0 59 0 1.46 80 214 160 150 0 100 140 0 158 0 1.384 80 214 489 627 0 100 815 0 704 0 1.461 80 227 3907 3779 0

Total number of rows: 43200

Table truncated, full table size 5557 Kbytes.




Supplementary data files not provided

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