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Sample GSM13294 Query DataSets for GSM13294
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 15
Sample type RNA
 
Channel 1
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_020
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.189062734 100 42 0 43 0 1.618 80 322 78 198 0 100 98 0 114 0 1.508 80 318 523 1099 0 100 271 0 346 0 1.495 80 318 1894 4505 0
A#10 -0.084981788 100 44 0 43 0 1.29 80 278 196 299 0 100 97 0 105 0 1.212 80 278 1239 1741 0 110 284 0 311 0 1.239 80 296 4903 7300 0
A#100 100 46 0 46 0 2.092 80 240 12 215 0 100 108 0 122 0 2.059 80 266 104 1296 0 100 298 0 390 0 1.933 80 240 374 5309 0
A#1000 100 53 0 50 0 3.925 80 237 2 0 -50 100 149 0 141 0 3.088 80 237 0 6 -50 100 458 0 431 0 3.646 80 228 31 61 -50
A#10000 100 53 0 48 0 1.498 80 216 72 199 0 100 152 0 134 0 1.411 80 230 429 1162 0 100 510 0 423 0 1.385 80 216 1667 4918 0
A#10001 100 55 0 48 0 1.796 80 231 50 123 0 100 154 0 129 0 1.704 80 231 309 726 0 100 514 0 415 0 1.588 80 237 1161 3201 0
A#10002 -0.548657244 110 57 0 51 0 1.393 80 222 109 278 0 110 161 0 140 0 1.372 80 236 695 1622 0 110 566 0 464 0 1.354 80 256 2668 6986 0
A#10003 0.077928924 100 57 0 53 0 1.293 80 226 112 160 0 100 164 0 140 0 1.261 80 236 773 930 0 90 614 0 517 0 1.314 52 244 2952 4044 0
A#10004 -0.10404172 110 55 0 52 0 1.174 80 233 470 576 0 110 165 0 138 0 1.156 80 224 2976 3265 0 110 562 0 479 0 1.155 80 254 11454 14134 0
A#10005 -0.611467522 100 57 0 53 0 1.548 80 237 67 214 0 100 181 0 150 0 1.411 80 223 410 1232 0 100 594 0 500 0 1.358 80 223 1642 5290 0
A#10006 -0.062634888 100 56 0 52 0 1.217 80 237 399 473 0 100 170 0 146 0 1.183 80 237 2647 2741 0 100 556 0 488 0 1.172 80 232 9667 11386 0
A#10007 0.054377722 100 56 0 50 0 1.173 80 238 296 325 0 110 163 0 138 0 1.197 80 222 1941 1869 0 100 533 0 437 0 1.191 80 218 7381 8080 0
A#10008 0.007495437 100 58 0 51 0 1.249 80 225 156 221 0 100 168 0 138 0 1.248 80 214 982 1217 0 100 551 0 449 0 1.219 80 221 3767 5044 0
A#10009 100 56 0 52 0 2.105 80 233 10 206 0 100 163 0 131 0 1.975 80 251 97 1243 0 100 555 0 454 0 1.961 80 253 282 5023 0
A#1001 0.047911338 110 51 0 48 0 1.353 80 230 101 127 0 110 137 0 129 0 1.314 80 237 649 728 0 110 425 0 405 0 1.313 80 236 2510 3050 0
A#10010 -0.52787669 100 56 0 51 0 1.218 80 258 240 474 0 110 163 0 129 0 1.185 80 265 1429 2588 0 100 525 0 440 0 1.222 80 258 5460 11589 0
A#10011 -0.773529589 100 57 0 51 0 1.506 80 292 62 232 0 100 163 0 134 0 1.459 80 260 349 1339 0 100 528 0 453 0 1.411 80 264 1459 5735 0
A#10012 60 57 0 52 0 1.34 32 146 51 148 0 60 162 0 136 0 1.48 32 134 331 932 0 60 561 0 459 0 1.452 32 134 1062 3512 0
A#10013 100 59 0 50 0 1.359 80 247 87 106 0 100 165 0 132 0 1.345 80 235 583 606 0 100 587 0 461 0 1.345 80 256 2286 2480 0
A#10014 0.301017062 100 60 0 51 0 1.38 80 228 128 139 0 100 168 0 132 0 1.289 80 222 793 782 0 100 583 0 465 0 1.327 80 228 2967 3399 0

Total number of rows: 43200

Table truncated, full table size 5534 Kbytes.




Supplementary data files not provided

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