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Sample GSM13295 Query DataSets for GSM13295
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 16
Sample type RNA
 
Channel 1
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_021
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.129321017 100 46 0 41 0 1.743 80 296 66 89 0 100 111 0 89 0 1.656 80 292 347 503 0 100 445 0 244 0 1.671 80 304 1598 1975 0
A#10 0.102860132 100 45 0 42 0 1.234 80 278 142 192 0 100 109 0 93 0 1.315 80 278 717 1090 0 110 410 0 274 0 1.306 80 304 3629 4511 0
A#100 90 56 0 53 0 2.941 52 238 15 40 0 60 185 0 172 0 2.967 32 152 77 262 0 60 777 0 683 0 3.252 32 138 458 763 0
A#1000 90 53 0 49 0 2.875 52 232 11 9 -50 90 139 0 132 0 3.6 52 220 55 62 -50 90 560 0 421 0 2.912 52 233 196 238 -50
A#10000 100 50 0 48 0 1.6 80 238 59 56 0 100 139 0 114 0 1.752 80 230 286 316 0 100 548 0 375 0 1.612 80 226 1280 1281 0
A#10001 90 51 0 47 0 2.033 52 245 21 30 -50 90 131 0 114 0 2.155 52 245 106 198 -50 90 547 0 385 0 2.393 52 233 394 740 -50
A#10002 0.220497399 100 51 0 48 0 1.865 80 276 46 64 0 100 144 0 124 0 1.859 80 280 236 349 0 100 579 0 393 0 1.807 80 258 1219 1588 0
A#10003 0.082054863 100 51 0 48 0 1.614 80 246 53 65 0 110 152 0 126 0 1.69 80 293 257 362 0 100 559 0 417 0 1.629 80 254 1311 1531 0
A#10004 0.141946823 100 52 0 48 0 1.168 80 212 377 440 0 100 148 0 132 0 1.172 80 249 2040 2612 0 100 577 0 426 0 1.134 80 245 9633 10654 0
A#10005 0.829404206 120 53 0 51 0 2.042 120 230 62 88 0 110 153 0 144 0 1.803 80 268 361 546 0 110 619 0 473 0 1.719 80 262 1605 2284 0
A#10006 0.346066067 100 52 0 49 0 1.355 80 213 128 171 0 100 140 0 129 0 1.392 80 240 674 1000 0 100 580 0 433 0 1.444 80 235 2941 4168 0
A#10007 0.285121729 100 54 0 49 0 1.228 80 222 257 326 0 100 134 0 121 0 1.172 80 226 1279 1879 0 100 554 0 380 0 1.194 80 237 6517 7868 0
A#10008 0.021720246 100 52 0 47 0 1.317 80 257 147 170 0 100 132 0 122 0 1.266 80 236 798 957 0 100 538 0 397 0 1.306 80 268 3604 3875 0
A#10009 80 52 0 47 0 1.877 52 200 24 42 0 100 136 0 123 0 2.129 80 246 117 225 0 80 563 0 394 0 2.63 52 225 545 1049 0
A#1001 0.415800825 110 54 0 47 0 1.625 80 286 57 108 0 110 140 0 121 0 1.646 80 266 313 619 0 110 567 0 373 0 1.514 80 265 1430 2601 0
A#10010 -0.283712075 100 53 0 49 0 1.394 80 266 122 113 0 100 143 0 118 0 1.34 80 242 639 646 0 100 556 0 382 0 1.4 80 258 2926 2789 0
A#10011 0.068044642 100 52 0 48 0 1.361 80 244 85 111 0 100 149 0 121 0 1.452 80 241 480 601 0 100 540 0 384 0 1.421 80 240 2363 2574 0
A#10012 60 51 0 47 0 1.878 32 139 34 38 0 70 149 0 118 0 2.285 32 170 178 217 0 60 517 0 374 0 2.027 32 142 620 840 0
A#10013 100 51 0 48 0 1.72 80 290 60 70 0 100 132 0 116 0 1.558 80 298 285 410 0 100 487 0 367 0 1.591 80 287 1583 1720 0
A#10014 0.03774225 100 50 0 47 0 1.72 80 252 56 65 0 100 124 0 111 0 1.638 80 230 296 407 0 100 475 0 363 0 1.873 80 239 1420 1600 0

Total number of rows: 43200

Table truncated, full table size 5469 Kbytes.




Supplementary data files not provided
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