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Sample GSM13296 Query DataSets for GSM13296
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 21
Sample type RNA
 
Channel 1
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 6 animals (No. N1, N2, N3, N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool3 starved = 6 animals (No. St1, St2, St3, St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_022
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.029391202 100 42 0 39 0 1.941 80 305 40 45 0 100 88 0 84 0 1.814 80 308 210 245 0 100 253 0 240 0 1.939 80 308 1029 1043 0
A#10 -0.268178887 110 44 0 42 0 2.208 80 328 23 29 0 100 94 0 84 0 2.156 80 278 126 168 0 100 286 0 262 0 1.962 80 278 584 730 0
A#100 0.142107856 90 46 0 43 0 2.302 52 299 24 29 0 100 104 0 91 0 2.181 80 278 152 149 0 90 335 0 302 0 2.272 52 233 690 651 0
A#1000 90 49 0 44 0 2.888 52 244 -1 -1 -50 90 117 0 94 0 3.384 52 244 3 3 -50 90 366 0 297 0 3.103 52 244 70 5 -50
A#10000 -0.332756201 80 57 0 49 0 1.63 52 173 47 60 0 90 157 0 142 0 1.672 52 237 231 316 0 100 548 0 461 0 1.774 80 216 1064 1284 0
A#10001 -0.406629153 70 66 0 59 0 2.59 32 180 28 33 0 70 210 0 169 0 2.933 32 158 169 196 0 100 582 0 449 0 1.607 80 212 894 1198 0
A#10002 -0.004292178 110 60 0 53 0 1.436 80 317 80 82 0 100 158 0 132 0 1.435 80 254 494 486 0 100 553 0 420 0 1.464 80 223 2029 2054 0
A#10003 0.004415669 100 57 0 50 0 1.403 80 298 79 75 0 90 153 0 127 0 1.389 52 281 459 499 0 100 553 0 397 0 1.459 80 285 1870 1828 0
A#10004 -0.001975247 110 57 0 50 0 1.249 80 280 379 346 0 110 164 0 133 0 1.253 80 300 2045 2077 0 110 620 0 403 0 1.48 80 274 8910 8445 0
A#10005 90 59 0 50 0 2.259 52 241 27 39 -50 90 166 0 132 0 2.249 52 241 192 226 -50 90 636 0 439 0 1.84 52 254 850 892 -50
A#10006 0.393970582 100 57 0 49 0 1.205 80 246 290 226 0 100 162 0 126 0 1.237 80 271 1673 1355 0 110 587 0 420 0 1.205 80 274 7268 5489 0
A#10007 0.08157543 110 56 0 49 0 1.257 80 228 297 274 0 110 161 0 126 0 1.203 80 249 1636 1542 0 110 544 0 417 0 1.296 80 230 7230 6476 0
A#10008 -0.203185601 100 56 0 49 0 1.367 80 247 119 134 0 100 153 0 120 0 1.369 80 232 738 790 0 100 542 0 415 0 1.348 80 231 2854 3102 0
A#10009 90 54 0 49 0 2.357 52 261 13 15 -50 90 149 0 119 0 3.481 52 257 37 107 -50 90 540 0 416 0 3.102 52 257 173 425 -50
A#1001 -0.023121259 100 47 0 43 0 2.277 80 301 28 26 0 100 105 0 89 0 2.024 80 311 141 163 0 100 284 0 265 0 2.061 80 281 728 631 0
A#10010 -0.40170071 100 56 0 48 0 1.244 80 271 158 188 0 100 147 0 116 0 1.269 80 271 884 1083 0 100 541 0 385 0 1.289 80 271 3763 4425 0
A#10011 0.098915961 90 58 0 49 0 1.479 52 213 78 72 0 90 147 0 119 0 1.596 52 213 432 426 0 90 541 0 375 0 1.604 52 213 1840 1803 0
A#10012 60 58 0 50 0 1.774 32 152 60 54 0 60 151 0 126 0 1.708 32 157 331 293 0 60 509 0 421 0 1.558 32 157 1477 1310 0
A#10013 -0.280535867 60 63 0 52 0 2.906 32 138 16 31 0 100 149 0 119 0 2.273 80 276 125 196 0 90 528 0 387 0 2.436 52 285 425 651 -50
A#10014 -0.29935808 100 59 0 51 0 1.953 80 278 52 62 0 100 162 0 130 0 1.842 80 246 298 386 0 100 602 0 437 0 1.833 80 298 1289 1427 0

Total number of rows: 43200

Table truncated, full table size 5462 Kbytes.




Supplementary data files not provided

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