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Sample GSM13298 Query DataSets for GSM13298
Status Public on Feb 09, 2004
Title liver, 24h starved, chip 23
Sample type RNA
 
Channel 1
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 6 animals (No. N1, N2, N3, N4, N5 and N6) fed during and before the 24h experiment with standard food, ad libitum; pool3 starved = 6 animals (No. St1, St2, St3, St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 24h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp4_024
 
Submission date Dec 05, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE853 liver, 24h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.303694287 100 39 0 42 0 1.943 80 334 35 51 0 100 72 0 87 0 1.758 80 348 185 301 0 100 211 0 279 0 1.535 80 344 935 1219 0
A#10 -0.276702471 110 42 0 45 0 1.223 80 307 151 176 0 110 88 0 103 0 1.289 80 321 695 1053 0 110 261 0 348 0 1.301 80 293 3795 4273 0
A#100 90 47 0 49 0 2.676 52 257 9 22 -50 90 105 0 131 0 3.405 52 281 20 119 -50 90 407 0 464 0 2.791 52 268 125 685 -50
A#1000 90 57 0 55 0 2.445 52 221 29 21 -50 90 141 0 155 0 2.603 52 221 138 125 -50 90 594 0 527 0 1.722 52 233 764 577 -50
A#10000 -0.248952365 100 64 0 58 0 1.469 80 235 89 117 0 100 197 0 169 0 1.41 80 235 489 665 0 100 719 0 608 0 1.343 80 230 2472 2699 0
A#10001 -0.074178321 80 67 0 59 0 1.975 52 209 46 52 0 90 206 0 179 0 1.653 52 244 215 287 -50 80 751 0 645 0 1.592 52 196 1053 1273 0
A#10002 0.074931695 100 67 0 59 0 1.927 80 260 58 62 0 90 213 0 181 0 2.149 52 244 276 329 -50 50 1100 0 860 0 1.516 12 97 1219 1845 0
A#10003 0.00215635 100 66 0 58 0 1.336 80 243 146 151 0 100 226 0 180 0 1.333 80 280 693 877 0 100 845 0 653 0 1.303 80 321 3775 3480 0
A#10004 0.192750476 100 69 0 59 0 1.223 80 232 467 402 0 100 228 0 177 0 1.154 80 232 2503 2387 0 110 802 0 629 0 1.169 80 216 11563 9379 0
A#10005 0.231550389 100 71 0 61 0 1.617 80 232 91 84 0 90 225 0 196 0 1.558 52 217 492 522 0 100 837 0 673 0 1.35 80 211 2267 2184 0
A#10006 0.208181008 110 69 0 60 0 1.187 80 244 538 453 0 110 223 0 191 0 1.256 80 273 2710 2666 0 100 804 0 652 0 1.163 80 236 13459 10706 0
A#10007 0.267624076 100 69 0 59 0 1.325 80 241 317 261 0 100 223 0 181 0 1.292 80 227 1609 1580 0 100 812 0 632 0 1.217 80 222 7682 5977 0
A#10008 -0.084555713 110 71 0 62 0 1.29 80 255 203 208 0 110 234 0 195 0 1.266 80 249 1062 1250 0 100 896 0 674 0 1.262 80 240 4626 5017 0
A#10009 60 79 0 66 0 2.161 32 140 52 63 0 90 243 0 194 0 1.617 52 244 223 378 -50 90 984 0 738 0 1.54 52 277 1347 1550 -50
A#1001 0.333571784 100 54 0 54 0 1.393 80 277 126 123 0 100 133 0 145 0 1.35 80 257 589 703 0 110 514 0 496 0 1.326 80 280 3579 2800 0
A#10010 -0.286711017 110 75 0 63 0 1.343 80 331 208 248 0 100 247 0 193 0 1.292 80 282 1069 1436 0 100 899 0 679 0 1.267 80 283 5395 5690 0
A#10011 0.074365051 90 70 0 60 0 1.275 52 221 188 185 0 90 230 0 183 0 1.288 52 234 922 1045 0 80 870 0 684 0 1.283 52 220 4461 4302 0
A#10012 90 73 0 62 0 3.442 52 289 7 14 -50 90 229 0 179 0 3.406 52 257 52 114 -50 90 867 0 669 0 3.312 52 282 297 432 -50
A#10013 0.35167031 50 86 0 83 0 2.752 12 78 53 61 0 100 238 0 191 0 2.133 80 242 210 281 0 100 915 0 678 0 2.214 80 244 873 1140 0
A#10014 0.258230388 100 74 0 60 0 1.324 80 250 177 167 0 100 233 0 184 0 1.33 80 239 941 998 0 100 885 0 669 0 1.237 80 227 4517 3724 0

Total number of rows: 43200

Table truncated, full table size 5530 Kbytes.




Supplementary data files not provided

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