|
| Status |
Public on Jan 01, 2015 |
| Title |
Reduction Mammoplasty [20130313_205635470814_12-21633-A2_08.RCC] |
| Sample type |
RNA |
| |
|
| Source name |
Reduction Mammoplasty
|
| Organism |
Homo sapiens |
| Characteristics |
age: NA
tumor size: NA
rfs event: NA
rfs time: NA
pam50: Normal
pam50+claudin-low: Normal
ror-s score: -1.21134478372997
ror-s groups: low
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from macrodissected FFPE tissue using ROCHE High Pure FFPE Kit
|
| Label |
NA
|
| Label protocol |
RNA was not labeled; instead, RNA (100 ng/ul) was pre-processed following the NanoString manufacturer's protocol by VHIO´s Translational Genomics Group
|
| |
|
| Hybridization protocol |
RNA was hybridized to capture probes and detection probes using the NanoString nCounter system according to the manufacturer's protocol. Detection probes were provided by the manufacturer and were labeled with a proprietary fluorescent barcode system
|
| Scan protocol |
Scanning was conducted using the NanoString confocal microscope scanner/Digital Analyzer according to manufacturer's instructions
|
| Data processing |
The data were normalized using 5 HK genes. Raw values are the number of copies of a feature-specific molecular barcode that is attached to a detection probe and counted by a confocal system
|
| |
|
| Submission date |
Feb 20, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Aleix Prat |
| E-mail(s) |
[email protected]
|
| Organization name |
VHIO
|
| Department |
Translational Genomics
|
| Street address |
Passeig Vall d´Hebron 119
|
| City |
Barcelona |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17071 |
| Series (1) |
| GSE55224 |
A Gene Expression-based Predictor of Fibroepithelial Lesions of the Breast |
|
