|
| Status |
Public on Mar 14, 2014 |
| Title |
AM14-1C4 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
AGM stromal clone
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: (CBAxC57BL/10)F1
tissue: AGM
age: embryonic day 11
hspc support: supportive
|
| Treatment protocol |
Stromal cells were cultured on 0.1% gelatin coated 12 well plates at 33°C. At confluency, culture medium was removed and cultures were washed in PBS 1X and total RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene from Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix).
|
| Description |
AM14-1C4-2
Gene expression from AGM supportive stromal clone.
|
| Data processing |
The data were RMA normalized and analysed with Partek Genomic Suite.
|
| |
|
| Submission date |
Mar 14, 2014 |
| Last update date |
Jun 20, 2014 |
| Contact name |
Florent Dumont |
| E-mail(s) |
[email protected]
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE44181 |
A systems biology approach for defining the molecular framework of the hematopoietic stem cell niche |
|
