|
| Status |
Public on Sep 15, 2006 |
| Title |
KOfurB_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
M. tuberculosis culture furB mutant
|
| Organism |
Mycobacterium tuberculosis |
| Characteristics |
Strain H37Rv
|
| Biomaterial provider |
Our own laboratory
|
| Treatment protocol |
Culture grew in physiological condition (7H9 ADC Middlebrook)
|
| Growth protocol |
Mid log phase culture was centrifuged and the pellet frozen with dry ice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Manganelli et al. Mol Micro (1999) 31:715-724
|
| Label |
Cy3
|
| Label protocol |
www.cag.icph.org
|
| |
|
| Channel 2 |
| Source name |
M. tuberculosis culture H37Rv
|
| Organism |
Mycobacterium tuberculosis |
| Characteristics |
Strain H37Rv
|
| Biomaterial provider |
Our own laboratory
|
| Treatment protocol |
Culture grew in physiological condition (7H9 ADC Middlebrook)
|
| Growth protocol |
Mid log phase culture was centrifuged and the pellet frozen with dry ice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Manganelli et al. Mol Micro (1999) 31:715-724
|
| Label |
Cy5
|
| Label protocol |
www.cag.icph.org
|
| |
|
| |
| Hybridization protocol |
www.cag.icph.org
|
| Scan protocol |
Prepared slides were immediately scanned using a 428TMArray Scanner (Affimetrix).
Fluorescence intensities of Cy3 and Cy5 dies at each spot were quantified using the ImaGene software 5.0 (BioDiscovery, Inc.)
|
| Description |
Synthesis of labelled samples was performed as described in protocols of the Center for Applied Genomics (www.cag.icph.org)
|
| Data processing |
Data obtained from qualified spots on the chip were normalised using the print-tip lowes implementation included in GEPAS v1.1 (http://gepas.bioinfo.cnio.es/). The values are expressed as ratio Cy5/Cy3 spots intensity determined from the normalized fluorescence intensity.
|
| |
|
| Submission date |
Sep 11, 2006 |
| Last update date |
Sep 14, 2006 |
| Contact name |
Elisa Dainese |
| E-mail(s) |
[email protected]
|
| Organization name |
Univerity of Padua
|
| Department |
Microbiology and virology
|
| Lab |
Prof. Manganelli
|
| Street address |
Via Gabelli 63
|
| City |
Padova |
| State/province |
Veneto |
| ZIP/Postal code |
35100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL4057 |
| Series (1) |
|
