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Sample GSM135863 Query DataSets for GSM135863
Status Public on Sep 13, 2006
Title D. sechellia pure-species, D. sechellia pure-species adults, genome chip, replicate 1, Cy3
Sample type RNA
 
Source name Drosophila sechellia males, 4 days old
Organism Drosophila sechellia
Characteristics Drosophila sechellia
Extracted molecule total RNA
Extraction protocol RNA isolation was accomplished with Qiagen RNeasy Mini Kit reagents. The RNA (10 µg) was annealed at the Duke Microarray Core Facility.
Label Cy3
Label protocol The labelling was done at the Duke Microarray Core Facility.
 
Scan protocol Fluorescent array images were collected at the Duke Microarray Core Facility.
Description D. sechellia pure-species, D. sechellia pure-species adults, genome chip
Data processing The median pixel feature intensity after background subtraction (EXPRESSION column) was log2 transformed, and normalized on the median for that hybridization (giving the VALUE column) to obtain the values given in the data table.
 
Submission date Sep 12, 2006
Last update date Jun 12, 2013
Contact name Amanda J. Moehring
E-mail(s) [email protected]
Organization name Duke University
Department DCMB/Biology
Lab Mohamed Noor
Street address Box 91000
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL3829
Series (2)
GSE5607 Comparison of D. simulans/D. sechellia F1 male hybrid genome-wide expression values to pure-species parents
GSE5655 Comparison of pure-species Drosophila expression to hybrid expression

Data table header descriptions
ID_REF clone number for genes with expression in Channel 1
EXPRESSION The channel 2 median pixel feature intensity after background subtraction
VALUE The channel 1 median pixel feature intensity after background subtraction

Data table
ID_REF EXPRESSION VALUE
1773 90 0.710493382
4472 50 -0.137503524
14407 4142 6.234752126
604 166 1.593679717
13564 114 1.0515303
3422 44 -0.321928095
2669 11 -2.321928095
9292 64 2.19E-01
5201 78 5.04E-01
11062 93 0.757799097
1560 53 -0.053439259
5089 68 0.306103127
6766 119 1.113458049
1131 77 0.485426827
4379 17 -1.693896873
11156 40 -0.459431619
9798 100 0.862496476
4531 62 0.172836596
8880 32 -0.781359714
11455 3400 5.949959316

Total number of rows: 15552

Table truncated, full table size 313 Kbytes.




Supplementary data files not provided

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