|
| Status |
Public on Nov 12, 2014 |
| Title |
Embryo 1.5 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Embryo culture
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Single Blastomere
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction was done following standard 24sure v3 protocol. In brief, 2.5µl extraction buffer and 5µl extraction cocktail were added per sample, followed by 10min incubation at 75°C and 4min at 95°C.
|
| Label |
cy3
|
| Label protocol |
Labelling was done following standard 24sure v3 protocol. In brief, samples were mixed with 5µl of random primers and denaturated for 5min at 94°C. Afterwards, samples were mixed with 12µl of Cy3/Cy5 labelling master mix and incubated at 37°C for 4 hours.
|
| |
|
| Channel 2 |
| Source name |
female/male reference
|
| Organism |
Homo sapiens |
| Characteristics |
sample: reference
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction was done following standard 24sure v3 protocol. In brief, 2.5µl extraction buffer and 5µl extraction cocktail were added per sample, followed by 10min incubation at 75°C and 4min at 95°C.
|
| Label |
cy5
|
| Label protocol |
Labelling was done following standard 24sure v3 protocol. In brief, samples were mixed with 5µl of random primers and denaturated for 5min at 94°C. Afterwards, samples were mixed with 12µl of Cy3/Cy5 labelling master mix and incubated at 37°C for 4 hours.
|
| |
|
| |
| Hybridization protocol |
After ethanol precipitation, samples were dissolved in hybridization mix at 75°C for 10 minutes, and incubated overnight at 47°C on the arrays in closed incubation chambers with 50% formamide. The slides were washed 10min in 2xSSC/0.05% Tween20, 10 min 1xSSC, 5min 0.1%SSC at 60°C and 1min in 0.1%SSC at RT
|
| Scan protocol |
Agilent dual laser DNA microarray scanner G2566AA was used for scanning the arrays, data was stored as .TIFF files
|
| Description |
Embryo 1 cell 5
The reference samples are male or female reference DNA as provided by Illumina, as part of the standard 24sure package. The ‘combined’ file represents combined results against both references.
The sample data tables display the combined results against both references.
|
| Data processing |
.TIFF files were imported in, and processed by, BlueFuse Multi 3.1.
|
| |
|
| Submission date |
Apr 15, 2014 |
| Last update date |
Nov 13, 2014 |
| Contact name |
Kurt Jacobs |
| E-mail(s) |
[email protected]
|
| Organization name |
Vrije Universiteit Brussel (VUB)
|
| Department |
Faculty of Medicine and Pharmacy
|
| Lab |
Research Group Reproduction and Genetics
|
| Street address |
Laarbeeklaan 103
|
| City |
Brussels |
| ZIP/Postal code |
1090 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL17760 |
| Series (1) |
| GSE56799 |
Chromosome constitution in embryos generated after in vitro maturation in a culture system supplemented with cAMP modulators |
|
