|
| Status |
Public on May 16, 2015 |
| Title |
Rosette_TCP15EAR_BiolRep1 |
| Sample type |
RNA |
| |
|
| Source name |
Rosette, P15::TCP15-EAR
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype/variation: P15::TCP15-EAR (transgenic)
tissue: rosette
age: 25 days
biological replicate: 1
|
| Treatment protocol |
Complete rosettes were taken 25 days after germination.
|
| Growth protocol |
Plants were grown on soil at 22-24ºC under a long-day photoperiod (16 h of illumination by a mixture of cool-white and GroLux fluorescent lamps) at an intensity of approximately 100 µE m-2 s-1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the RNeasy Plant Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
The labelling protocol used was GE1 v6.5.
|
| |
|
| Hybridization protocol |
Hybridization was performed at 10 rpm for 17 hours at 65°C in a rotating Agilent hybridization oven.
|
| Scan protocol |
Slides were scanned on the Agilent DNA Microarray Scanner (G2505B-C).
|
| Description |
Leaves_TCP15-EAR
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol GE1_107_Sept09). Normexp background subtraction was applied followed by a quantile normalization.
|
| |
|
| Submission date |
May 16, 2014 |
| Last update date |
May 16, 2015 |
| Contact name |
Agustin Lucas Arce |
| E-mail(s) |
[email protected]
|
| Organization name |
Instituto de Agrobiotecnología del Litoral
|
| Lab |
Laboratorio de Agrobiotecnología
|
| Street address |
Ciudad Universitaria
|
| City |
Santa Fe |
| State/province |
Santa Fe |
| ZIP/Postal code |
3000 |
| Country |
Argentina |
| |
|
| Platform ID |
GPL12621 |
| Series (2) |
| GSE57742 |
Transcriptomic effects of the expression in Arabidopsis of a TCP15-EAR fusion controlled by its own promoter (one-color experiment) |
| GSE57744 |
Transcriptomic effects of the expression in Arabidopsis of a TCP15-EAR fusion controlled by its own promoter |
|
