|
| Status |
Public on Sep 10, 2008 |
| Title |
mPB CD34+CD38- cells, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
mobilized peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
CD34+CD38- cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy colums (Qiagen)
|
| Label |
biotin
|
| Label protocol |
biotinylated cRNA
|
| |
|
| Hybridization protocol |
following fragmentation 10 ug cRNA was hybridized at 45 C for 16 hours with constant rotation at 60 rpm
|
| Scan protocol |
Scanning was performed as described in the GeneChip Expression Analysis technical manual
|
| Description |
Gene expression data from CD34+CD38- cells isolated from normal human Bone Marrow
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
|
| |
|
| Submission date |
Oct 27, 2006 |
| Last update date |
Mar 01, 2008 |
| Contact name |
Arnolda de Nooij-van Dalen |
| Organization name |
LUMC
|
| Department |
Hematology
|
| Lab |
Experimental Hematology
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6146 |
Expression data from CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cellsmbryo |
|
