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| Status |
Public on Sep 10, 2008 |
| Title |
Expression data from CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cellsmbryo |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiles of CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cells were compared. Comparison of expression profiles of hematopoietic stem cells from fetal liver, umbilical cord blood, bone marrow and mobilized pheripheral blood allowed us to identify a unique set of genes with conserved expression during ontogeny.
Keywords: Cell type comparison
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| Overall design |
CD34+CD38- en CD34+CD38+ cell populations were isolated by cell sorting from human Bone Marrow, mobilized peripheral blood, umbilical cord blood and fetal liver. Total RNA was isolated from each cell population followed by the synthesis of biotinylated cRNA. After fragmentation the biotinylated cRNA was hybridized to affymetrix U133A chips.
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| Contributor(s) |
de Nooij-van Dalen A, Fibbe W, Scherjon S, Willemze R, Barge R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 27, 2006 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Arnolda de Nooij-van Dalen |
| Organization name |
LUMC
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| Department |
Hematology
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| Lab |
Experimental Hematology
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| Street address |
Albinusdreef 2
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| City |
Leiden |
| ZIP/Postal code |
2333 ZA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (14)
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| GSM142565 |
BM CD34+CD38- cells, biological replicate 1 |
| GSM142566 |
BM CD34+CD38+ cells, biological replicate 1 |
| GSM142567 |
mPB CD34+CD38- cells, biological replicate 1 |
| GSM142568 |
mPB CD34+CD38+ cells, biological replicate 1 |
| GSM142569 |
mPB CD34+CD38- cells, biological replicate 2 |
| GSM142570 |
mPB CD34+CD38+ cells, biological replicate 2 |
| GSM142571 |
UCB CD34+CD38- cells, biological replicate 1 |
| GSM142572 |
UCB CD34+CD38+ cells, biological replicate 1 |
| GSM142573 |
UCB CD34+CD38- cells, biological replicate 2 |
| GSM142574 |
UCB CD34+CD38+ cells, biological replicate 2 |
| GSM142575 |
FL CD34+CD38- cells, biological replicate 1 |
| GSM142576 |
FL CD34+CD38+ cells, biological replicate 1 |
| GSM142577 |
FL CD34+CD38- cells, biological replicate 2 |
| GSM142578 |
FL CD34+CD38+ cells, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA97683 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6146_RAW.tar |
28.0 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
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