|
| Status |
Public on Jun 30, 2015 |
| Title |
ZT62 |
| Sample type |
RNA |
| |
|
| Source name |
liver total RNA, ZT62
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
|
| Treatment protocol |
Food and water available ad libitum.
|
| Growth protocol |
Adults mice were housed under 1 12-h light/12-h dark regimen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each tissue sample was extracted using TRizol (Invitrogen) according to the manufacturer's instructions. Total RNA quality was assessed by Bioanalyzer 2100 (Agilent). Total RNA of each time point were mixed in the equal amount into one pooled sample.
|
| Label |
Cy3
|
| Label protocol |
Labeling and hybridization of total RNA were performed according to the manufacturer’s protocol with no modification.
|
| |
|
| Hybridization protocol |
Same as above. The entire reaction was hybridized to the array for 20 hours at 55°C.
|
| Scan protocol |
Microarray results were extracted using Agilent Microarray Scanner (Agilent)
|
| Description |
ZT62
|
| Data processing |
The miRNA signals and errors on miRNA microarray were obtained from gTotalGeneSignal and gTotalGeneError respectively in the output files of Agilent Feature Extraction software (v9.5.3.1), subsequently analyzed by Gene-Spring GX 7.3.1 software (Agilent).
|
| |
|
| Submission date |
Jul 15, 2014 |
| Last update date |
Jun 30, 2015 |
| Contact name |
Haifang Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
PICB
|
| Department |
functional genomics group
|
| Street address |
NO 320, Yueyang Road
|
| City |
ShangHai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL10384 |
| Series (1) |
| GSE59423 |
Post-transcriptional regulation of circadian rhythm by miRNA in mouse liver |
|
