|
| Status |
Public on Jul 28, 2014 |
| Title |
Donor BB - HIN1 - 4 HAU/ml - mAdbID:111301 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pooled PBMC Reference
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: peripheral blood mononuclear cells (PBMCs)
other: Sample Pooling Protocol: A pool of total RNA derived from independent human PBMCs served as a common reference.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample Extraction Protocol
Other: Total RNA was isolated from virus-stimulated samples and untreated controls with Qiagen RNeasy Plus microkits (Qiagen, Valencia, CA).
|
| Label |
cy3
|
| Label protocol |
Cy3 Sample Labeling
Other: Samples were directly labeled with the Kreatech (Durham, NC) ULS aRNA fluorescent labeling kit.
|
| |
|
| Channel 2 |
| Source name |
Donor BB - H1N1 - 4 HAU/ml
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: Plasmacytoid dendritic cells (pDCs)
individual: Donor BB
|
| Treatment protocol |
Treatment dose: 4 HAU/ml
Treatment time: 6 hours
In-vitro treatment: Human plasmacytoid dendritic cells (pDCs) were isolated by negative selection from PBMCs of healthy blood donors and cultured for 18 hours prior to infection. pDCs were stimulated for 6 hours with influenza virus A/PR/8/34 (H1N1; Charles River Laboratories) at 4 HAU/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample Extraction Protocol
Other: Total RNA was isolated from virus-stimulated samples and untreated controls with Qiagen RNeasy Plus microkits (Qiagen, Valencia, CA).
|
| Label |
cy5
|
| Label protocol |
Cy5 Sample Labeling
Other: Samples were directly labeled with the Kreatech (Durham, NC) ULS aRNA fluorescent labeling kit.
|
| |
|
| |
| Hybridization protocol |
Hybrization Protocol
Other: Hybridization was carried out in a water bath at 42C for 18 hours.
|
| Scan protocol |
Scan_MicronsPerPixelX: 10
Scan_MicronsPerPixelY: 10
Scan_ScannerName: Agilent Technologies Scanner G2505B US45103062
|
| Description |
mAdb experiment ID: 111301
|
| Data processing |
Agilent Data Processing Protocol
Calculation Method: Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction Software. Spot values were normalized using the default linear-lowess normalization.
FeatureExtractor_Version: 9.5.1.1
Protocol_Name: GE2-NonAT_95_Feb07 (Read Only)
|
| |
|
| Submission date |
Jul 28, 2014 |
| Last update date |
Jul 28, 2014 |
| Contact name |
Francesco Maria Marincola |
| E-mail(s) |
[email protected]
|
| Phone |
301-793-8210
|
| Organization name |
Sidra Medical and Research Center
|
| Street address |
Al Nasr Tower, AL Corniche Street, PO Box 26999
|
| City |
Doha |
| ZIP/Postal code |
PO Box 26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL7088 |
| Series (1) |
| GSE59837 |
Differential Responses of Plasmacytoid Dendritic Cells to Influenza and Distinct Viral Pathogens |
|
