|
| Status |
Public on Aug 01, 2015 |
| Title |
Muscle_BA_180_rep2_0713 |
| Sample type |
RNA |
| |
|
| Source name |
Longissimus thoracis muscle, BA, 180 dpc
|
| Organism |
Bos taurus |
| Characteristics |
tissue: Longissimus thoracis muscle
breed: Blonde Aquitaine
developmental stage: fetus
gestational stage: 180dpc
animal number: 0713
mother number: 225
father name: Fallou
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Purelink RNA Mini Kit (Invitrogen) followed by a Dnase treatment (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
50 ng of total RNA extracted were amplified and fluorescently labelled to produce Cy3 cRNA using the Low Input Quick Amp Labeling one color Kit (Agilent).
|
| |
|
| Hybridization protocol |
After purification (RNeasy Mini Kit, Qiagen), 1650 ng of labeled cRNA were hybridized onto G2519F Bovine GE 4x44K v2 microarray (Agilent) with hybridization buffer (Gene Expression Hybridization Kit, Agilent) according to the manufacturer’s instructions. After 17h of hybridization, slides were washed with the Gene Expression Wash Pack (Agilent).
|
| Scan protocol |
Microarrays were scanned with Agilent G2505 scanner.
|
| Description |
Biological replicate 2 of 2
|
| Data processing |
Data were extracted and normalized with Feature Extraction 10.7 software (Agilent) using background and multiplicative detrend (no background subtraction). The mean intensity between the technical replicates was then calculated for each probe.
|
| |
|
| Submission date |
Aug 27, 2014 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Isabelle Cassar-Malek |
| Organization name |
Inra
|
| Lab |
UMR1213 Herbivores
|
| Street address |
Research centre Clermont-Ferrand-Theix-Lyon
|
| City |
Saint-Genès-Champanelle |
| ZIP/Postal code |
63122 |
| Country |
France |
| |
|
| Platform ID |
GPL11649 |
| Series (1) |
| GSE60844 |
Molecular regulation of enhanced muscle mass in developing Blond Aquitaine foetuses compared to Charolais |
|
