|
| Status |
Public on Apr 30, 2015 |
| Title |
AD_17 adult animal, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
AD_17
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
strain: Wistar
age: Adult
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
|
| Label |
biotin
|
| Label protocol |
Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from adult animals
|
| Data processing |
The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of GeneChip® Rat Gene 1.0 from Affymetrix. A Custom CDF Version 18 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
|
| |
|
| Submission date |
Sep 01, 2014 |
| Last update date |
Apr 30, 2015 |
| Contact name |
Harsh Dweep |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Heidelberg
|
| Department |
Medical Research Center, Medical Faculty of Mannheim of the University of Heidelberg
|
| Street address |
Theodor-Kutzer-Ufer 1-3
|
| City |
Mannheim |
| State/province |
baden wurttemberg |
| ZIP/Postal code |
68135 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17896 |
| Series (2) |
| GSE60961 |
Alteration of mRNA and microRNA expression profiles in rat muscular type vasculature in early postnatal development [mRNA] |
| GSE60963 |
Alteration of mRNA and microRNA expression profiles in rat muscular type vasculature in early postnatal development |
|
