|
| Status |
Public on Sep 30, 2014 |
| Title |
Affected 1 |
| Sample type |
genomic |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Schizophrenia
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from whole blood using the 5 Prime Perfect Pure DNA Blood Kit (Gaithersburg, MD, USA), following the manufacturer’s protocol
|
| Label |
Cy3, Cy5
|
| Label protocol |
Immunoprecipitation of methylated DNA was performed using BiomagTM magnetic beads coupled mouse monoclonal antibody against 5-methylcytidine. The immunoprecipitated DNA was eluted and purified by phenol chloroform extraction and ethanol precipitation.
|
| |
|
| Hybridization protocol |
The total input and immunoprecipitated DNA were labeled with Cy3- and Cy5-labeled random 9-mers, respectively, and hybridized to NimbleGen Human DNA Methylation 3x720K Promoter Plus CpG Island Arrays
|
| Scan protocol |
Scanning was performed with the Axon GenePix 4000B microarray scanner
|
| Description |
Processed at ArrayStar
|
| Data processing |
Data was processed using Partek Genomics Suite
|
| |
|
| Submission date |
Sep 29, 2014 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Christina A Castellani |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Western Ontario
|
| Department |
Biology
|
| Street address |
Biological and Geological Sciences Building
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A5B7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17148 |
| Series (1) |
| GSE61862 |
DNA Methylation Across Monozygotic Twin Pairs and Their Parents |
|
