|
| Status |
Public on Nov 07, 2014 |
| Title |
GNMT knockout at 3 month age, biological replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver, GNMT knockout, non-alcoholic steatohepatitis, 3 months
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57B6SJL
genotype/variation: GNMT knockout
age: 3 months
tissue: liver
disease status: non-alcoholic steatohepatitis
|
| Treatment protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
2-4 mg of liver. The tissue received was pulverized in mortar with presence of liquid nitrogen. Total RNA was then extracted using Qiagen’s RNeasy Mini kit recovering each sample in 35μl of water.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Affymetrix Mouse430_2.na21 platform. GeneChips were washed and stained in the Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
GNMT_3ko4
Gene expression data from GNMT knockout at 3 month, when the tissue has been histopathologically determined to be non-alcoholic steatohepatitis.
|
| Data processing |
The data were acquired with GCOS instrument and processed using RMA normalization method.
|
| |
|
| Submission date |
Nov 05, 2014 |
| Last update date |
Nov 07, 2014 |
| Contact name |
Itziar Frades |
| Organization name |
Swedish University of Agricultural Sciences
|
| Department |
Plant Protection Biology
|
| Street address |
Sundsvägen 14
|
| City |
Alnarp |
| ZIP/Postal code |
SE-230 53 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE63027 |
Expression data from GNMT and MAT1A knockout models that develop all the stages of non-alcoholic fatty liver disease including hepatocellular carcinoma [GNMT_MAT1A_3&8_months] |
| GSE63068 |
Integrative genomic signatures of hepatocellular carcinoma derived from nonalcoholic fatty liver disease |
|
