|
| Status |
Public on Dec 03, 2015 |
| Title |
PDGF+p53-/-RBPjk-/- tumor rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PDGF+p53-/-RBPjk-/- tumor
|
| Organism |
Mus musculus |
| Characteristics |
genotype pdgfb gene: over expressed
genotype trp53: KO
genotype rbpj gene: KO
tissue: brain tumor
|
| Treatment protocol |
Mice were euthanized and their brains were isolated. Brain tumors were identified by green fluorescent protein (GFP) expression from a Cre-inducible GFP reporter, micro-dissected, mechanically dissociated and placed in Trizol solution.
|
| Growth protocol |
Brain tumors were genetically induced in transgenic mice. Tissue was harvested when mice showed behavioral symptoms of tumor formation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol by ATLAS Biolabs GmbH, Germany
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Mouse Gene 1.0 ST Array was used. Standard Affymetrix protocol by ATLAS Biolabs GmbH, Germany
|
| Scan protocol |
Standard Affymetrix protocol by ATLAS Biolabs GmbH, Germany
|
| Data processing |
Arrays were normalized by RMA (oligo package from Bioconductor).
|
| |
|
| Submission date |
Dec 16, 2014 |
| Last update date |
Nov 17, 2021 |
| Contact name |
DBM Bioinformatics Core Facility |
| Phone |
+41612073541
|
| Organization name |
University of Basel
|
| Department |
Departement of Biomedicine
|
| Street address |
Hebelstrasse 20
|
| City |
Basel |
| State/province |
BS |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE64230 |
Expression data from murine brain tumors |
|
