|
| Status |
Public on Jun 08, 2015 |
| Title |
PD_Fb_SNCA-ExCont RNAi |
| Sample type |
RNA |
| |
|
| Source name |
ND27760
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
age: 55YR
tissue: fibroblast
|
| Treatment protocol |
Cells were treated by SNCA expression-control RNAi.
|
| Growth protocol |
Cells were grown in Minimum Essential Medium (MEM) supplemented with 10% fetal bovine serum at 37ºC in 5% CO2 humidified chamber.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from treated fibroblasts using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
PD fibroblasts were treated by SNCA expression-control RNAi.
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals. Reference Web link: http://www.3d-gene.com
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| |
|
| Submission date |
Dec 17, 2014 |
| Last update date |
Jun 08, 2015 |
| Contact name |
Hirohiko Hohjoh |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Neuroscience, NCNP
|
| Street address |
4-1-1 Ogawahigashi
|
| City |
Kodaira |
| State/province |
Tokyo |
| ZIP/Postal code |
187-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE64266 |
Gene expression profiles of PD fibroblasts treated by SNCA expression-control RNAi. |
|
