|
| Status |
Public on Jan 31, 2007 |
| Title |
Calu3-GemR_G_4 |
| Sample type |
RNA |
| |
|
| Source name |
Calu3 human non-small lung cancer cells, gemcitabine resistant (Ligand)
|
| Organism |
Homo sapiens |
| Characteristics |
gemcitabine resistant Calu3 derivative generated from the parental Calu3 line by consecutive treatments with 50 nM gemcitabine over a period of 3 months
|
| Biomaterial provider |
Ligand Pharmaceuticals, San Diego, CA
|
| Treatment protocol |
gemcitabine resistant Calu3 were treated over 10 consecutive 10-day treatment cycles with 50 nM gemcitabine for the first 3 days of the cycle followed by 7 days of vehicle
|
| Growth protocol |
cells were cultured in RPMI 1640 supplemented with 10% FBS and 2 mM glutamine in 95% air, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy column (Qiagen) purification of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 701021 Revision 4, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner 2500.
|
| Description |
gemcitabine resistant Calu3 cells, treated for 10 cycles of 10 days each with gemcitabine (still gemcitabine resistant)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
|
| |
|
| Submission date |
Jan 30, 2007 |
| Last update date |
Jan 30, 2007 |
| Contact name |
Thomas Hermann |
| E-mail(s) |
[email protected]
|
| Organization name |
Ligand Pharmaceuticals
|
| Department |
Molecular Oncology
|
| Street address |
10275 Science Center Dr.
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6914 |
Gene expression associated with gemcitabine resistance and its reversal by bexarotene |
|
