|
| Status |
Public on Feb 04, 2015 |
| Title |
SVC_NCD_Day0_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Stromal vascular fraction_NCD_Day0
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: Epididymal adipose tissue
treatment: normal chow
time: 0 d
|
| Treatment protocol |
Mice were fed normal chow diet (NCD) until they were subjected to 60% HFD for the indicated time periods.
|
| Growth protocol |
Seven-week-old male C57BL/6J mice were obtained from The Jackson Laboratory and were housed in colony cages in 12-h light/12-h dark cycles.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Epididymal adipopse tissues were chopped and incubated in collagenase buffer. After centrifugation, adipocytes in the supernatant and pelleted stromal vascular cell (SVC) fraction were collected for RNA extraction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (GeneChip® WT Terminal Labeling and Controls Kit P/N 901525 1, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, fragmented cDNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Mouse Gene 1.0 ST Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Short-term HFD
|
| Data processing |
The Robust Multiarray Average (RMA) algorithm implemented in Affymetrix Expression Console software (version1.3.1) was used to normalize the raw data. Highly expressed genes that showed over 2-fold difference between the signal values of control and test samples were selected for further study.
|
| |
|
| Submission date |
Feb 03, 2015 |
| Last update date |
Feb 04, 2015 |
| Contact name |
Jae Bum Kim |
| E-mail(s) |
[email protected]
|
| Phone |
+82-2-880-4409
|
| Organization name |
Seoul National University
|
| Department |
School of Biological Sciences
|
| Lab |
Lab. of Adipocyte and Metabolism Research
|
| Street address |
Bldg 105-Rm. 333, Seoul National University, San 56-1, Sillim Dong, Kwanak-Gu
|
| City |
Seoul |
| ZIP/Postal code |
156-742 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE65557 |
Microarray analysis of Adipose tissue of Short-term HFD-fed mice |
|
