|
| Status |
Public on Mar 25, 2016 |
| Title |
HepaRG_6h_C3D3B3G1 |
| Sample type |
RNA |
| |
|
| Source name |
HepaRG Hepatocytes
|
| Organism |
Homo sapiens |
| Characteristics |
chemical treatment: Ethenyl Estradiol
dose: 10 nM
replicate: 3
gender: Female
cell line: HepaRG
|
| Treatment protocol |
Cells in collagen coated 24 well plates (BD, Franklin Lakes, NJ) were treated with 3 concentrations of chemical or DMSO vehicle for 6 h.
|
| Growth protocol |
HepaRG human hepatoma cells were purchased from Biopredic International (Rennes, France). Differentiated HepaRG cells were grown following suppliers’ protocol (Biopredic) in Williams E medium (Invitrogen), supplemented with 2mM Glutamax (Invitrogen), 10% ADD670 supplement (Biopredic International) and maintained at 37°C in an atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were extracted in QIAgen RLT buffer and total RNA was extracted using the Agencourt RNAdvance Tissue protocol implemented on a Beckman FXp robot using methods provided by Beckman Coulter.
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was converted to Affymetrix GeneChip targets using the Affymetrix IVT-Express kit and protocols provided by Affymetrix.
|
| |
|
| Hybridization protocol |
GeneChip targets were hybridized on Affymetrix U219-96 plates using the GeneTitan single-channel instrument and protocols provided by Affymetrix.
|
| Scan protocol |
GeneTitan plates were scanned on a GeneTitan single-channel instrument using protocols provided by Affymetrix.
|
| Description |
Chemical signature/dose response study
|
| Data processing |
Data was processed using the PLIER algorithm implemented from the Affymetrix Command Console Software.
|
| |
|
| Submission date |
Jun 12, 2015 |
| Last update date |
Mar 26, 2016 |
| Contact name |
Jay Patrick Tiesman |
| Organization name |
Procter & Gamble
|
| Department |
Global Biotechnology
|
| Lab |
Mason Business Center
|
| Street address |
PO BOX 8006
|
| City |
MASON |
| State/province |
OH |
| ZIP/Postal code |
45040-8006 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (2) |
| GSE69844 |
Expression data for HepaRG cells treated with 34 different chemicals |
| GSE69851 |
Human toxicology-relevant cell lines treated with three doses of 34 chemical compounds |
|
