|
| Status |
Public on Mar 10, 2007 |
| Title |
433 (4.5K BAC array) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Primary Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Age Diagnosis: 1083, INSS Stage:4, MYCN:Amplified, 1p36 LOH:LOH, 11q23 LOH:No LOH, Unb11qLOH:No Unbalanced LOH, 17q gain:Unbalanced Gain, Tumor %:100Event Free Survival=event, Outcome=Death
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen DNA extraction standard protocol
|
| Label |
Cy3
|
| Label protocol |
Random primer labeling kit (Invitrogen)
|
| |
|
| Channel 2 |
| Source name |
Primary Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Age Diagnosis: 1083, INSS Stage:4, MYCN:Amplified, 1p36 LOH:LOH, 11q23 LOH:No LOH, Unb11qLOH:No Unbalanced LOH, 17q gain:Unbalanced Gain, Tumor %:100Event Free Survival=event, Outcome=Death
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen DNA extraction standard protocol
|
| Label |
Cy5
|
| Label protocol |
Random primer labeling kit (Invitrogen)
|
| |
|
| |
| Hybridization protocol |
48-72 h at 37 deg C on a slowly rotating platform. Slide washing was done using published protocols (Hodgson et al. 2001)
|
| Scan protocol |
Affymetrix 428 microarray scanner and analyzed with Genepix software (Axon)
|
| Description |
Primary neuroblastoma tumor.
|
| Data processing |
Normalized by global print-tip adjustment of the median dye ratio(Cy3/Cy5), quality filtered, replicate spots and dye swaps merged
|
| |
|
| Submission date |
Mar 09, 2007 |
| Last update date |
Mar 14, 2007 |
| Contact name |
Jayanti Jagannathan |
| E-mail(s) |
[email protected]
|
| Phone |
2674262286
|
| Organization name |
Childrens Hospital of Philadelphia
|
| Department |
Oncology
|
| Street address |
34th & Civic center blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL4894 |
| Series (1) |
| GSE7230 |
Genome-wide assessment of DNA aberrations in human neuroblastoma primary tumors using BAC array |
|
| Relations |
| Alternative to |
GSM90389 (gene expression data from the same sample) |
