|
| Status |
Public on May 22, 2007 |
| Title |
Family B/II_3 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
DNA isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
aunt of an autistic patient (analysed in channel 2)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by means of Qiagen Genomic Tip columns
|
| Label |
Cy3
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| Channel 2 |
| Source name |
DNA isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
female reference DNA from a healthy donor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by means of Qiagen Genomic Tip columns
|
| Label |
Cy5
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| |
| Hybridization protocol |
Hybridization was done overnight at 42C in a slide booster (Advalytix, Brunnthal, Germany). Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| Scan protocol |
Slides were scanned using an Agilent scanner (PMT settings: 100%/100%)
|
| Description |
Family B/II_3
|
| Data processing |
TIFF images were analysed by Genepix 5.0 (Axon Instruments, Union City, CA) and raw intensities (gpr files) were imported into CGHPRO (Chen at al.,2005). Background intensities were not substracted. Signal intensities were normalized by subgridd LOWESS. Aberrant ratios involving three or more neighbouring clones wereconsidered as genomic aberrations, unless they coincided with a known polymorphism as listed in the Database of Genomic Variants (version Dec 2005)
|
| |
|
| Submission date |
Apr 24, 2007 |
| Last update date |
May 22, 2007 |
| Contact name |
Reinhard Ullmann |
| E-mail(s) |
[email protected]
|
| Phone |
00493084131251
|
| Organization name |
MPIMG
|
| Department |
Human Molecular Genetics
|
| Lab |
Molecular Cytogenetics
|
| Street address |
Ihnestr.73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5114 |
| Series (1) |
| GSE6225 |
16p13 aberrations predispose to autism and MR |
|
