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Platform GPL5114 Query DataSets for GPL5114
Status Public on May 22, 2007
Title MPIMG Homo sapiens 36k_arrayCGH8
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Homo sapiens
Manufacturer MPIMG_BERLIN
Manufacture protocol The submegabase resolution whole genome tiling path BAC array consists of the human 32k BAC Re-Array Set* (BACPAC Resources Center, DNA kindly provided by Nigel Carter, Wellcome Trust Sanger Centre) and a set if subtelomeric clones (assembled by members of the COSTB19 Action: Molecular cytogenetics of solid tumours). Protocols for the isolation of DNA (1 Mb Sanger set and subtelomeric set) and the amplification of insert DNA by linker adapter PCR are described in detail at the Molecular Cytogenetics website of MPG. Amplicons were ethanol precipitated, dissolved in 3XSSC/1,5M betaine and spotted on epoxy-coated slides (NUNC, Wiesbaden, FRG) by means of a modified Qarray (originally from Genetix, new Milton, U.K.) and Pointech (Gibbon,MN) Tungston PTL 2500 slit pins. Each BAC is represented by a single spot on the array. * due to recurrent abnormal ratios in a series of control hybridisation encompassing 30 tumors, clones from plate HGP DNA RP C 12_2 were excluded from further analysis.
Support glass
Coating other
 
 
Submission date Apr 23, 2007
Last update date May 27, 2007
Contact name Reinhard Ullmann
E-mail(s) [email protected]
Phone 00493084131251
Organization name MPIMG
Department Human Molecular Genetics
Lab Molecular Cytogenetics
Street address Ihnestr.73
City Berlin
ZIP/Postal code 14195
Country Germany
 
Samples (179) GSM183680, GSM183681, GSM183682, GSM183683, GSM183684, GSM183685 
Series (13)
GSE6225 16p13 aberrations predispose to autism and MR
GSE7527 Array CGH in congenital heart disease
GSE7886 A defect in the ionotropic glutamate receptor 6 gene (GRIK2) causes autosomal recessive mental retardation

Data table header descriptions
ID
Internal_Name clone name for internal use
Name original library name
Block block
Row row
Col column
Chromosom chromosome
Chromosom_start clone startposition (UCSC Maz 2004(Hg17),NCBI Build 35)
Chromosom_end clone end position (UCSC Maz 2004(Hg17),NCBI Build 35)
GB_RANGE NCBI Build 35 chr accession [start..end]
CLONE_ID BAC Clone
SPOT_ID spot identifier

Data table
ID Internal_Name Name Block Row Col Chromosom Chromosom_start Chromosom_end GB_RANGE CLONE_ID SPOT_ID
1_1_1_bK1444N24 bK1444N24 bK1444N24 1 1 1 -- -- -- bK1444N24
1_1_2_Buffer_PCR Buffer_PCR Buffer_PCR 1 1 2 -- -- -- --Buffer_PCR
1_1_3_397B22 397B22 397B22 1 1 3 -- -- -- 397B22
1_1_4_Buffer_PCR Buffer_PCR Buffer_PCR 1 1 4 -- -- -- --Buffer_PCR
1_1_5_Buffer_PCR Buffer_PCR Buffer_PCR 1 1 5 -- -- -- --Buffer_PCR
1_1_6_F0488J12 F0488J12 RP13-488J12 1 1 6 chr6 39981664 40077232 NC_000006.9[39981664..40077232] RP13-488J12
1_1_7_N0413M21 N0413M21 RP11-413M21 1 1 7 chr2 49722275 49893627 NC_000002.9[49722275..49893627] RP11-413M21
1_1_8_D2542F24 D2542F24 CTD-2542F24 1 1 8 chrX 142934882 143104925 NC_000023.8[142934882..143104925] CTD-2542F24
1_1_9_N0137H06 N0137H06 RP11-137H6 1 1 9 chr7 20092898 20246525 NC_000007.11[20092898..20246525] RP11-137H6
1_1_10_N0673B19 N0673B19 RP11-673B19 1 1 10 chr1 170769040 170971853 NC_000001.8[170769040..170971853] RP11-673B19
1_1_11_N0090E12(del) N0090E12(del) N0090E12(del) 1 1 11 -- -- -- N0090E12(del)
1_1_12_N0097G03 N0097G03 RP11-97G3 1 1 12 chr5 169019435 169186042 NC_000005.8[169019435..169186042] RP11-97G3
1_1_13_N1030C19 N1030C19 RP11-1030C19 1 1 13 chr15 67512352 67703569 NC_000015.8[67512352..67703569] RP11-1030C19
1_1_14_N0345M21 N0345M21 RP11-345M21 1 1 14 chr14 34291680 34493927 NC_000014.7[34291680..34493927] RP11-345M21
1_1_15_N0281A03 N0281A03 RP11-281A3 1 1 15 chr15 94793749 94982390 NC_000015.8[94793749..94982390] RP11-281A3
1_1_16_N0771D21 N0771D21 RP11-771D21 1 1 16 chr6 52037630 52237235 NC_000006.9[52037630..52237235] RP11-771D21
1_1_17_N0504D21 N0504D21 RP11-504D21 1 1 17 chr11 41145833 41340204 NC_000011.8[41145833..41340204] RP11-504D21
1_1_18_N0490A23 N0490A23 RP11-490A23 1 1 18 chr14 19164830 19301441 NC_000014.7[19164830..19301441] RP11-490A23
1_1_19_M2112G04 M2112G04 CTD-2112G4 1 1 19 chr11 26048566 26161137 NC_000011.8[26048566..26161137] CTD-2112G4
1_1_20_N0282F14 N0282F14 RP11-282F14 1 1 20 chr3 176949096 176992855 NC_000003.9[176949096..176992855] RP11-282F14

Total number of rows: 41760

Table truncated, full table size 4365 Kbytes.






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