|
| Status |
Public on Dec 01, 2015 |
| Title |
DLBCL_DS-66_Specimen USC77 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Human DLBCL specimen
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: diffuse large B-cell lymphoma
treatment: R-CHOP or R-CHOP like immuno-chemo therapy
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA was performed using Qiagen DNeasy Blood and Tissue kit according to the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Genomic DNA samples were labeled using the Agilent Genomic DNA Enzymatic Labeling Kit, according to the manufacturer's protocol, except that 1μg of DNA was used per 25μl labeling reaction mix.
|
| |
|
| Channel 2 |
| Source name |
Reference human genome DNA equimixture of normal male and normal female (Promega).
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA was performed using Qiagen DNeasy Blood and Tissue kit according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Genomic DNA samples were labeled using the Agilent Genomic DNA Enzymatic Labeling Kit, according to the manufacturer's protocol, except that 1μg of DNA was used per 25μl labeling reaction mix.
|
| |
|
| |
| Hybridization protocol |
Oligoarray hybridization buffer, block (both from Agilent In Situ Hybridization Kit Plus), and Cot-1 DNA (Life Technologies) were added to each sample prior to application to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization for 24 hours, slides were washed sequentially as described by the manufacturer.
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505B.
|
| Description |
DLBCL_DS-66_Specimen USC77 as test DNA and Promega normal DNA as reference
|
| Data processing |
Data extraction was performed using Feature Extraction Version 10.7.3.1 (Agilent Technologies).
|
| |
|
| Submission date |
Oct 14, 2015 |
| Last update date |
Dec 01, 2015 |
| Contact name |
Jane Houldsworth |
| E-mail(s) |
[email protected]
|
| Phone |
2015289171
|
| Organization name |
Cancer Genetics, Inc.
|
| Street address |
201 Route 17 North
|
| City |
Rutherford |
| State/province |
NJ |
| ZIP/Postal code |
07070 |
| Country |
USA |
| |
|
| Platform ID |
GPL21031 |
| Series (1) |
| GSE74025 |
Cross-Platform Assessment of Genomic Imbalance Confirms the Clinical Relevance of Genomic Complexity and Reveals Loci with Potential Pathogenic Roles in Diffuse Large B-Cell Lymphoma |
|
