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Sample GSM1908348 Query DataSets for GSM1908348
Status Public on Dec 01, 2015
Title DLBCL_DS-66_Specimen USC82
Sample type genomic
 
Channel 1
Source name Human DLBCL specimen
Organism Homo sapiens
Characteristics disease state: diffuse large B-cell lymphoma
treatment: R-CHOP or R-CHOP like immuno-chemo therapy
Extracted molecule genomic DNA
Extraction protocol Extraction of genomic DNA was performed using Qiagen DNeasy Blood and Tissue kit according to the manufacturer's instructions.
Label Cy5
Label protocol Genomic DNA samples were labeled using the Agilent Genomic DNA Enzymatic Labeling Kit, according to the manufacturer's protocol, except that 1μg of DNA was used per 25μl labeling reaction mix.
 
Channel 2
Source name Reference human genome DNA equimixture of normal male and normal female (Promega).
Organism Homo sapiens
Characteristics sample type: reference
Extracted molecule genomic DNA
Extraction protocol Extraction of genomic DNA was performed using Qiagen DNeasy Blood and Tissue kit according to the manufacturer's instructions.
Label Cy3
Label protocol Genomic DNA samples were labeled using the Agilent Genomic DNA Enzymatic Labeling Kit, according to the manufacturer's protocol, except that 1μg of DNA was used per 25μl labeling reaction mix.
 
 
Hybridization protocol Oligoarray hybridization buffer, block (both from Agilent In Situ Hybridization Kit Plus), and Cot-1 DNA (Life Technologies) were added to each sample prior to application to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization for 24 hours, slides were washed sequentially as described by the manufacturer.
Scan protocol Scanned on an Agilent Technologies Scanner G2505B.
Description DLBCL_DS-66_Specimen USC82 as test DNA and Promega normal DNA as reference
Data processing Data extraction was performed using Feature Extraction Version 10.7.3.1 (Agilent Technologies).
 
Submission date Oct 14, 2015
Last update date Dec 01, 2015
Contact name Jane Houldsworth
E-mail(s) [email protected]
Phone 2015289171
Organization name Cancer Genetics, Inc.
Street address 201 Route 17 North
City Rutherford
State/province NJ
ZIP/Postal code 07070
Country USA
 
Platform ID GPL21031
Series (1)
GSE74025 Cross-Platform Assessment of Genomic Imbalance Confirms the Clinical Relevance of Genomic Complexity and Reveals Loci with Potential Pathogenic Roles in Diffuse Large B-Cell Lymphoma

Data table header descriptions
ID_REF
VALUE log2 Cy5/Cy3

Data table
ID_REF VALUE
A_16_P15000916 0.119279563
A_16_P15003416 -0.314927367
A_16_P21686635 0.697002448
A_16_P21686737 0.385854391
A_16_P30009023 0.040252392
A_18_P10003549 -0.134436116
A_16_P00000776 0.081012302
A_18_P10003754 -0.252864416
A_16_P15003873 0.186047047
A_16_P15003937 0.175090179
A_16_P00000964 -0.498010984
A_16_P30010422 0.092185402
A_16_P30010606 -0.702916933
A_16_P35004752 -0.004093632
A_16_P15004272 -0.23061289
A_16_P00001153 -0.585616957
A_16_P00001230 -0.013650506
A_18_P10004493 0.086975427
A_18_P10005642 -0.07398445
A_16_P00001417 0.435834251

Total number of rows: 23683

Table truncated, full table size 639 Kbytes.




Supplementary file Size Download File type/resource
GSM1908348_USC_82_SG13284320_256689910067_S001_CGH_1105_Oct12_2_2.txt.gz 5.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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