|
| Status |
Public on Nov 24, 2018 |
| Title |
bin2-1 |
| Sample type |
SRA |
| |
|
| Source name |
Arabidopsis thaliana_root_bin2-1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genetic background: Columbia
genotype: bin2-1
tissue: root
|
| Treatment protocol |
treated with or without 100 μM ABA for 3 h
|
| Growth protocol |
Arabidopsis Col, bin2-1 and bzr1-D/bin2-1 were grown vertically on 1/2 MS in growth chamber (Percival) at 22 degree under long-day (16h of light/8 h of dark) for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a RNeasy Plant Mini Kit (Qiagen)
The mRNA sequencing libraries were constructed with barcodes using the TruSeq RNA Sample Preparation Kit (Illumina),Six barcoded libraries were pooled together and sequenced by Illumina HiSeq2000
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
processed data file: bzr1-D/bin2-1 vs bin2-1 original data.xls; bin2-1_vs_Col-ABA_original_data.xls
|
| Data processing |
The sequence reads were mapped to the Arabidopsis genome (TAIR 10) using Tophat software
Differential gene expression was analyzed using Cufflink software
Genome_build: TAIR 10
Supplementary_files_format_and_content: excel files include averaged FPKM value for each sample. Differential expression analysis was performed using the program cufflinks
|
| |
|
| Submission date |
Nov 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Wenqiang Tang |
| E-mail(s) |
[email protected]
|
| Organization name |
Hebei Normal University
|
| Department |
College of Life Sciences
|
| Street address |
East 20 NanErHuang Road
|
| City |
Shijiazhuang |
| State/province |
Hebei Province |
| ZIP/Postal code |
050024 |
| Country |
China |
| |
|
| Platform ID |
GPL13222 |
| Series (1) |
| GSE75377 |
Antagonistic Regulation of Abscisic Acid Inhibited Root Growth By Brassinosteroid is Partially Mediated by BRASSINAZOLE RESISTENT 1 suppressed ABSCISIC ACID INSENSITIVE 5 expression |
|
| Relations |
| BioSample |
SAMN04293501 |
| SRA |
SRX1451701 |
