|
| Status |
Public on Feb 23, 2016 |
| Title |
K20 iPSC |
| Sample type |
RNA |
| |
|
| Source name |
LRRK2 patient line #2 fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Parkinsons disease
cell type: Human skin fibroblast
cell line: K20
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 5
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Feb 22, 2016 |
| Last update date |
Feb 23, 2016 |
| Contact name |
Ying Pei |
| Organization name |
Buck Institute
|
| Street address |
8001 redwood blvd
|
| City |
novato |
| State/province |
ca |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE78152 |
Derivation, characterization, and neuronal differentiation of integration-free induced pluripotent stem cell lines from Parkinsons disease patients carrying SNCA, LRRK2, PARK2, and GBA mutations |
|
