|
| Status |
Public on Feb 24, 2016 |
| Title |
ΔaceA PQ 1mM 2 |
| Sample type |
RNA |
| |
|
| Source name |
PQ 1mM
|
| Organism |
Pseudomonas aeruginosa PAO1 |
| Characteristics |
strain: ΔPA2634 transposon insertion mutant
treatment: 1mM PQ
|
| Treatment protocol |
Exponential phase cell was used for microarray analysis. In case of paraquat treatment, 1mM concentration of paraquat was added on exponential phase cell for 15min.
|
| Growth protocol |
Overnight culture was diluted 1:100 in 5 mL LB medium at 37°C with aeration.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Labeling reactions were performed with a Bioprime labeling kit (Invitrogen) in a volume of 50 μl with a modified dNTP pool containing 120 μM each of dATP, dGTP, and dCTP; 60 μM dTTP; and 60 μM Cy5-dUTP (for all)
|
| |
|
| Hybridization protocol |
Labeled cDNAs were mixed with hybridization solution (MYcroarray.com), and the hybridization mixtures were heated at 65℃ for 5 min and then immediately cooled on ice for 5 min. Hybridization mixtures were directly loaded onto assembled MYcroarray.com microarray. The arrays hybridized at 50℃ for 16 hr using Agilent Hybridization oven (Agilent Technology, USA).
|
| Scan protocol |
The hybridization images were analyzed by GenePix Pro 6.0 (Axon Instruments, CA).
|
| Data processing |
The average fluorescence intensity for each spot was calculated and local background was subtracted. All data normalization and selection of fold-changed genes were performed using GenoWiz 4.0 (Ocimumbiosolutions, India).
|
| |
|
| Submission date |
Feb 23, 2016 |
| Last update date |
Feb 24, 2016 |
| Contact name |
Sungeun Ahn |
| E-mail(s) |
[email protected]
|
| Organization name |
Korea university
|
| Department |
molecular microbiology
|
| Lab |
molecular environmental microbiology Laboratory
|
| Street address |
Anamro
|
| City |
Anam-Dong, Seungbuk-Ku |
| State/province |
Seoul |
| ZIP/Postal code |
02841 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL21507 |
| Series (1) |
| GSE78230 |
Gene expression-based investigation of a new role for the glyoxylate shunt in contributing to tolerance to oxidative stress. |
|
