|
| Status |
Public on Mar 10, 2018 |
| Title |
Lung_AEC_MyD88_KO_2h_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
AEC_KO_2h
|
| Organism |
Mus musculus |
| Characteristics |
background: C57BL/6
gender: male
tissue: Lung
cell type: Sorted Airway Epithelial Cells
timepoint: 2h
treatment: OVA/ stFLA
genotype: MyD88 -/-
|
| Treatment protocol |
Mice were sensitized oropharyngeally with 100 ug ovalbumin (OVA)/ 1250 ng standard flagellin (stFLA) for 2 hours in vivo. After this time, lungs were isolated and digested with elastase and DNase to generate a single cell suspension. Cells were stained for flow cytometry and sorted for 7-AAD- CD31- CD34- CD45- EpCAM+ cells to include AECs but not endothelial cells or leukocytes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from sorted AECs was isolated using the Trizol extraction protocol. RNA was concentrated and excess organic solvents removed using the Qiagen Rneasy MinElute Cleanup Kit.
|
| Label |
biotin
|
| Label protocol |
50 ng of each total RNA sample was labeled according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was done according to the manufacturer's instructions.
|
| Scan protocol |
Gene expression was quantified on the nCounter Digital AnalyzerTM.
|
| Description |
Gene expression from MyD88 -/- airway epithelial cells
|
| Data processing |
Raw data was normalized using quantile normalization.
|
| |
|
| Submission date |
Mar 24, 2016 |
| Last update date |
Mar 10, 2018 |
| Contact name |
Seddon Thomas |
| E-mail(s) |
[email protected]
|
| Organization name |
NIEHS
|
| Street address |
111 T. W. Alexander Dr.,
|
| City |
RTP |
| State/province |
North Carolina |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL19964 |
| Series (2) |
| GSE79591 |
Nanostring of sorted airway epithelial cells (AECs) from cell-specific MyD88 KO at 2h after in vivo sensitization with OVA/standard flagellin |
| GSE79615 |
MYD88-dependent dendritic and epithelial cell crosstalk in the lung orchestrates immune responses to inhaled allergens |
|
