|
| Status |
Public on Mar 23, 2017 |
| Title |
Control tBRD-2 RNAi replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
D. melanogaster testes
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: adult testis
genotype: +/+;tbrd-2RNAi/Sb
|
| Treatment protocol |
Testes from 0-2 day old control and tbrd-2 knockdown flies were dissected in PBS
|
| Growth protocol |
Drosophila melanogaster strains were maintained on standard medium at 30°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from control testes and tbrd-2 knockdown testes by using TRIzol® (Invitrogen). RNA quality was validated using the Agilent Bioanalyser 2100 with the RNA 6000 Nano kit. Gene expression analysis was carried out using Affymetrix Drosophila Genome 2.0 arrays. For each array, independent RNA from whole testes pooled from 30 animals was used.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the appropriate Affymetrix protocol without further amplification.
|
| |
|
| Hybridization protocol |
According to manufacturers instructions without modifications.
|
| Scan protocol |
According to manufacturers instructions without modifications.
|
| Description |
Undriven tbrd-2RNAi control males carried one copy of tbrd-2RNAi (III chromosome).
|
| Data processing |
Data processing was done in R. CEL files were parsed using the affy package, intensities were background corrected and normalized using the rma function with default settings.
|
| |
|
| Submission date |
May 02, 2016 |
| Last update date |
Mar 24, 2017 |
| Contact name |
Marek Bartkuhn |
| E-mail(s) |
[email protected]
|
| Organization name |
Justus-Liebig-University Giessen
|
| Department |
Biomedical Informatics and Systems Medicine
|
| Street address |
Aulweg 132
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE81019 |
Comparison of gene expression in control bam-Gal4 and control undriven tbrd-2RNAi Drosophila testes with tbrd-2 knockdown testes |
|
