|
| Status |
Public on May 03, 2016 |
| Title |
control P14 sample #81 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampal microgila PND14-control
|
| Organism |
Mus musculus |
| Characteristics |
tissue: hippocampus
cell type: microglia
gender: male
strain: Balbc
age: PND14
|
| Treatment protocol |
Mice were raised under control condition as described previously (Wei L. et al. Developmental neuroscience, 2015).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
microglia were acutely isolated from the hippocampus using Percoll gradient followed by FACS analysis. RNA was harvested using the he mirVana mirRNA isolation kit (Cat # AM 1560, Life technologies) and was then concentrated and Dnase treated on column using the RNA clean and contractor-5 kit (Cat# R1015, Zymo Research).
|
| Label |
nanostring fluorescence barcode
|
| Label protocol |
Two ng of RNA were converted to cDNA using Superscript Vilo (Cat# 11754, Life Technologies), amplified with 5 rounds of PCR according to the nCounter single cell gene expression protocol and hybridized to the mouse immune panel (Cat# 150761, NanoString Technologies).
|
| |
|
| Hybridization protocol |
Samples were processed for hybridization using automated nCounter Prep Station.
|
| Scan protocol |
Hybridized complexes were scanned in the nCounter Digital analyzer.
|
| Data processing |
Counts were normalized to positive controls and 5 housekeeping genes (e.g. Alas1, Gapdh, Oaz1, Polr1b, Sdha, Tbp) that were not affected by either BDS or age and showed a coefficient of variation of less than 20%. Genes were considered “present” if at least 40% of the samples showed hybridization signal that was 2 s.d. above the mean value for the negative control probes.
|
| |
|
| Submission date |
May 02, 2016 |
| Last update date |
May 03, 2016 |
| Contact name |
Arie Kaffman |
| E-mail(s) |
[email protected]
|
| Phone |
2037856657
|
| Organization name |
Yale University
|
| Department |
Psychiatry
|
| Lab |
Kaffman
|
| Street address |
300 George St. Suite 901
|
| City |
New Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL19964 |
| Series (2) |
| GSE81035 |
Early life stress perturbs the maturation of microglia in the developing hippocampus [development] |
| GSE81038 |
Early life stress perturbs the maturation of microglia in the developing hippocampus |
|
