|
| Status |
Public on Dec 25, 2008 |
| Title |
Schizophrenia patient 1356 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
DNA isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
school performance: no low school result
severity of illness: severe
family history: yes
age of onset: 24 yr
tissue: blood
disease state: Schizophrenia.
gender: female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by a standard phenol-chloroform protocol.
|
| Label |
Cy3
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics, http://www.molgen.mpg.de/~abt_rop/molecular_cytogenetics/ArrayCGH2006.html)
|
| |
|
| Channel 2 |
| Source name |
DNA isolated from blood (reference)
|
| Organism |
Homo sapiens |
| Characteristics |
sample: DNA pool of 50 healthy females.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by a standard phenol-chloroform protocol.
|
| Label |
Cy5
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics, http://www.molgen.mpg.de/~abt_rop/molecular_cytogenetics/ArrayCGH2006.html)
|
| |
|
| |
| Hybridization protocol |
Hybridization was done overnight at 42°C in a slide booster (Advalytix, Brunnthal, Germany). Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics, http://www.molgen.mpg.de/~abt_rop/molecular_cytogenetics/ArrayCGH2006.html)
|
| Scan protocol |
Slides were scanned using an Agilent scanner, PMT settings:100/100%.
|
| Description |
Array CGH analysis of a patient with Schizophrenia
|
| Data processing |
TIFF images were analysed by Genepix 5.0 (Axon Instruments, Union City, CA) and raw intensities (gpr files) were imported into CGHPRO (Chen at al.,2005). Background intensities were not substracted. Signal intensities were normalized by subgridd LOWESS. Aberrant ratios involving three or more neighbouring clones wereconsidered as genomic aberrations, unless they coincided with a known polymorphism as listed in the Database of Genomic Variants (version Dec 2005)
|
| |
|
| Submission date |
Aug 01, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Reinhard Ullmann |
| E-mail(s) |
[email protected]
|
| Phone |
00493084131251
|
| Organization name |
MPIMG
|
| Department |
Human Molecular Genetics
|
| Lab |
Molecular Cytogenetics
|
| Street address |
Ihnestr.73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5114 |
| Series (1) |
| GSE8606 |
Comparative genomic hybridization suggests a role for NRXN1 and APBA2 in schizophrenia |
|
